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Peptide Receptors in Intestinal Epithelium

Marc Laburthe, Brigitte Amiranoff

10.1002/cphy.cp060211

Source: Supplement 17: Handbook of Physiology, The Gastrointestinal System, Neural and Endocrine Biology

Originally published: 1989

Published online: January 2011

Full Article on Wiley Online Library



Abstract

The sections in this article are:

1 Receptor Concept
2 Tissue Preparation for Receptor Studies
3 Entire Mucosa
4 Isolated Epithelial Cells
5 Cultured Epithelial Cells
6 Established Peptide Receptors
6.1 Vasoactive Intestinal Peptide
6.2 Peptide YY and Neuropeptide Y
6.3 Somatostatin
6.4 Epidermal Growth Factor
6.5 Insulin
7 Candidate Peptide Receptors
7.1 Neurotensin
7.2 Opioid Peptides
8 Conclusion
Figure 1. Figure 1.

Transmission electron microscopy of postconfluent Caco‐2 cells. A: polarized cell monolayer with brush‐border and tight junctions. B: cross section of microvilli with core filaments. C: junctional complex of desmosome and tight junction.

From G. Chevalier, unpublished data.
Figure 2. Figure 2.

Morphology of mucus‐secreting clonal HT‐29/16E cell line. A: low‐magnification electron micrograph of a vertical section of postconfluent cells showing polarized mucus secretion. B: ultrastructure of a mucus‐secreting cell exhibiting features of a typical goblet cell; vertical section. C: electron microscopy of a section cut parallel to surface of cultured cells.

From Augeron and Laboisse 14
Figure 3. Figure 3.

Competitive inhibition of specific 125I‐labeled vasoactive intestinal peptide (VIP) binding to rat and human intestinal epithelial cell membranes by VIP and related peptides. hGRF, human growth hormone‐releasing factor; PHI, peptide histidine isoleucine amide; PHM, peptide histidine methionine; rGRF, rat growth hormone‐releasing factor; Sec, secretin.

Redrawn from Laburthe et al. 208,216,217
Figure 4. Figure 4.

Adenylate cyclase activity in rat and human intestinal epithelial cell membranes in response to vasoactive intestinal peptide (VIP) and related peptides. hGRF, human growth hormone‐releasing factor; PHI, peptide histidine isoleucine amide; PHM, peptide histidine methionine; rGRF, rat growth hormone‐releasing factor; Sec, secretin.

Redrawn from Laburthe et al. 208,216,217
Figure 5. Figure 5.

Correlation between specific binding of 125I‐labeled vasoactive intestinal peptide (VIP) and enzyme markers of different subcellular fractions of rat enterocytes.

From Dharmsathaphorn et al. 88
Figure 6. Figure 6.

Inhibition of 125I‐labeled peptide YY (PYY) binding to plasma membranes from rat intestinal epithelial cells by unlabeled PYY; neuropeptide Y (NPY); and human (h), rat (r), and avian (a) pancreatic polypeptide (PP).

From Laburthe et al. 214
Figure 7. Figure 7.

Stimulation of DNA synthesis (A) and cell division (B) by epidermal growth factor (EGF) in rat small intestinal cell line RIE‐1.

From Blay and Brown 30), reprinted by permission from The Biochemical Society, London
Figure 8. Figure 8.

Binding of 125I‐labeled epidermal growth factor (EGF) to cultured rat small intestinal cells (RIE‐1) as a function of 125I‐labeled EGF concentration.

From Blay and Brown 30), reprinted by permission from The Biochemical Society, London
Figure 9. Figure 9.

Light‐microscope autoradiographs of paraffin sections of villi of duodenum (A, B), jejunum (C, D), ileum (E, F), and crypts of colon (G, H) at 2 min after intravenous injection of 270.8 × 106 dpm of 125I‐labeled insulin (109 μCi/μg). Sections from experimental animals are noted in A, C, E, and G and from corresponding controls in which an additional 75 μg of unlabeled insulin was coinjected in B, D, F, and H. In sections from experimental animals, silver grains are revealed over lamina propria (LP) and columnar epithelium (Ep), especially nuclear regions of epithelium. Grains are absent over striated border (bb) as well as over mucous granules of goblet cells. In controls, a marked diminution in grain density over epithelium is noted, but grain density remains high over its LP. × 600.

From Bergeron et al. 20


Figure 1.

Transmission electron microscopy of postconfluent Caco‐2 cells. A: polarized cell monolayer with brush‐border and tight junctions. B: cross section of microvilli with core filaments. C: junctional complex of desmosome and tight junction.

From G. Chevalier, unpublished data.


Figure 2.

Morphology of mucus‐secreting clonal HT‐29/16E cell line. A: low‐magnification electron micrograph of a vertical section of postconfluent cells showing polarized mucus secretion. B: ultrastructure of a mucus‐secreting cell exhibiting features of a typical goblet cell; vertical section. C: electron microscopy of a section cut parallel to surface of cultured cells.

From Augeron and Laboisse 14


Figure 3.

Competitive inhibition of specific 125I‐labeled vasoactive intestinal peptide (VIP) binding to rat and human intestinal epithelial cell membranes by VIP and related peptides. hGRF, human growth hormone‐releasing factor; PHI, peptide histidine isoleucine amide; PHM, peptide histidine methionine; rGRF, rat growth hormone‐releasing factor; Sec, secretin.

Redrawn from Laburthe et al. 208,216,217


Figure 4.

Adenylate cyclase activity in rat and human intestinal epithelial cell membranes in response to vasoactive intestinal peptide (VIP) and related peptides. hGRF, human growth hormone‐releasing factor; PHI, peptide histidine isoleucine amide; PHM, peptide histidine methionine; rGRF, rat growth hormone‐releasing factor; Sec, secretin.

Redrawn from Laburthe et al. 208,216,217


Figure 5.

Correlation between specific binding of 125I‐labeled vasoactive intestinal peptide (VIP) and enzyme markers of different subcellular fractions of rat enterocytes.

From Dharmsathaphorn et al. 88


Figure 6.

Inhibition of 125I‐labeled peptide YY (PYY) binding to plasma membranes from rat intestinal epithelial cells by unlabeled PYY; neuropeptide Y (NPY); and human (h), rat (r), and avian (a) pancreatic polypeptide (PP).

From Laburthe et al. 214


Figure 7.

Stimulation of DNA synthesis (A) and cell division (B) by epidermal growth factor (EGF) in rat small intestinal cell line RIE‐1.

From Blay and Brown 30), reprinted by permission from The Biochemical Society, London


Figure 8.

Binding of 125I‐labeled epidermal growth factor (EGF) to cultured rat small intestinal cells (RIE‐1) as a function of 125I‐labeled EGF concentration.

From Blay and Brown 30), reprinted by permission from The Biochemical Society, London


Figure 9.

Light‐microscope autoradiographs of paraffin sections of villi of duodenum (A, B), jejunum (C, D), ileum (E, F), and crypts of colon (G, H) at 2 min after intravenous injection of 270.8 × 106 dpm of 125I‐labeled insulin (109 μCi/μg). Sections from experimental animals are noted in A, C, E, and G and from corresponding controls in which an additional 75 μg of unlabeled insulin was coinjected in B, D, F, and H. In sections from experimental animals, silver grains are revealed over lamina propria (LP) and columnar epithelium (Ep), especially nuclear regions of epithelium. Grains are absent over striated border (bb) as well as over mucous granules of goblet cells. In controls, a marked diminution in grain density over epithelium is noted, but grain density remains high over its LP. × 600.

From Bergeron et al. 20
References
 1. Albuquerque, R. H., C. W. I., Owens, and S. R. Bloom. A study of vasoactive intestinal polypeptide (VIP) stimulated intestinal fluid secretion in rat and its inhibition by indo‐methacin. Experientia Basel 35: 1496–1497, 1979.
 2. Allen, J. M., J. C., Yeats, T. E. Adrian, M. L. Fitzpatrick, and S. R. Bloom. Absorption of oral glucose load in man is delayed by peptide YY (Abstract). Diabetologia 27: 251A, 1984.
 3. Alumets, J., F., Sundler, and R. Håkanson. Distribution, ontogeny and ultrastructure of somatostatin immunoreactive cells in the pancreas and gut. Cell Tissue Res. 185: 465–479, 1977.
 4. Amiranoff, B., M., Laburthe, C. Dupont, and G. Rosselin. Characterization of a vasoactive intestinal peptide‐sensitive adenylate cyclase in rat intestinal epithelial cell membranes. Biochim. Biophys. Acta 544: 474–481, 1978.
 5. Amiranoff, B., M., Laburthe, and G. Rosselin. Characterization of specific binding sites for vasoactive intestinal peptide (VIP) in rat intestinal epithelial cell membranes. Biochim. Biophys. Acta 627: 215–224, 1980.
 6. Amiranoff, B., M., Laburthe, and G. Rosselin. Potentiation by guanine nucleotides of the VIP‐induced adenylate cyclase stimulation in intestinal epithelial cell membranes. Life Sci. 26: 1905–1911, 1980.
 7. Amiranoff, B., M., Laburthe, C. Rouyer‐Fessard, J. G. Demaille, and G. Rosselin. Regulation par la calmoduline de l'activité adenylate cyclasique de l'épithelium intestinal. C. R. Hebd. Seances Acad. Sci. 294: 487–489, 1982.
 8. Amiranoff, B., M., Laburthe, C. Rouyer‐Ressard, J. G. Demaille, and G. Rosselin. Calmodulin stimulation of intestinal epithelium adenylate cyclase. Eur. J. Biochem. 130: 33–37, 1983.
 9. Amiranoff, B., N., Vauclin‐Jacques, N. Boige, C. Rouyer‐Fessard, and M. Laburthe. Interaction of Gila monster venom with VIP receptors in intestinal epithelium of human: a comparison with rat. FEBS Lett. 164: 299–302, 1983.
 10. Andersson, S., D. Chang, K. Folkers, and S. Rossell. Inhibition of gastric acid secretion by neurotensin. Life Sci. 19: 367–370, 1976.
 11. Arilla, E., M. P., Lopez‐Ruiz, L. Gonzalez Guijarro, J. C. Prieto, A. Gomez‐Pan, and B. Hirst. Characterization of somatostatin binding sites in cytosolic fraction of rat intestinal mucosa. Biochim. Biophys. Acta 802: 203–208, 1984.
 12. Arimura, A. Recent progress in somatostatin research. Biomed. Res. 2: 233–257, 1981.
 13. Arimura, A., H., Sato, A. Dupont, N. Nishi, and A. Schally. Somatostatin: abundance of immunoreactive hormone in rat stomach and pancreas. Science Wash. DC 189: 1007–1009, 1975.
 14. Augeron, C., and C. Laboisse. Emergence of permanently differentiated cell clones in a human colonic cancer cell line in culture after treatment with sodium butyrate. Cancer Res. 44: 3961–3966, 1984.
 15. Barbezat, G. O., and M. I. Grossman. Intestinal secretion: stimulation by peptide. Science Wash. DC 174: 422–424, 1971.
 16. Barnes, D., and S. P. Colowick. Stimulation of sugar uptake in cultured fibroblasts by epidermal growth factor (EGF) and EGF‐binding arginine esterase. J. Cell. Physiol. 89: 633–640, 1976.
 17. Bastl, C. P., C. A., Barnett, T. J. Schmidt, and G. Litwack. Glucocorticoid stimulation of sodium absorption in colon epithelia is mediated by corticosteroid IB receptor. J. Biol. Chem. 259: 1186–1195, 1984.
 18. Bataille, D., C., Gespach, M. Laburthe, B. Amiranoff, K. Tatemoto, N. Vauclin, and G. Rosselin. Porcine peptide having N‐terminal histidine and C‐terminal isoleucine amide: VIP‐like and secretin‐like effects in different tissues from rat. FEBS Lett. 114: 240–242, 1980.
 19. Beaulieu, J. F., and R. Calvert. The effect of epidermal growth factor (EGF) on the differentiation of the rough endoplasmic reticulum in fetal mouse small intestine in organ culture. J. Histochem. Cytochem. 29: 765–770, 1981.
 20. Bergeron, J. J. M., R., Rachubinski, N. Searle, D. Borts, R. Sikstrom, and B. I. Posner. Polypeptide hormone receptors in vivo: demonstration of insulin binding to adrenal gland and gastrointestinal epithelium by quantitative radioautoradiography. J. Histochem. Cytochem. 28: 824–835, 1980.
 21. Besson, J., M., Laburthe, D. Bataille, C. Dupont, and G. Rosselin. Vasoactive intestinal peptide (VIP): tissue distribution in the rat as measured by radioimmunoassay and by radioreceptorassay. Acta Endocrinol. 87: 799–810, 1978.
 22. Besson, J., W., Rostene, A. M. Lhiaubet, B. Poussin, and G. Rosselin. Release of vasoactive intestinal peptide from rat jejuno‐ileum in vitro. Effect of various depolarizing agents. Experientia Basel 39: 732–733, 1983.
 23. Beubler, E. Influence of vasoactive intestinal polypeptide on net water flux and cyclic adenosine 3',5'‐monophosphate formation in the rat jejunum. Naunyn‐Schmiedeberg's Arch. Pharmacol. 313: 243–247, 1980.
 24. Beubler, E. VIP and PGE1 activate adenylate cyclase in rat intestinal cell membranes via different mechanism. Eur. J. Pharmacol. 74: 67–72, 1981.
 25. Beubler, E., and F. Lembeck. Inhibition of stimulated fluid secretion on the rat small and large intestine by opiates agonists. Naunyn‐Schmiedeberg's Arch. Pharmacol. 306: 113–118, 1979.
 26. Binder, A. J. Net fluid and electrolyte secretion: the pathophysiological basis for diarrhea. In: Mechanisms of Intestinal Secretion, edited by H. J. Binder. New York: Liss, 1979, p. 1–15. (Kroc Found. Ser. 12.)
 27. Binder, H. J., G. F., Lemp, and J. D. Gardner. Receptors for vasoactive intestinal peptide and secretin on small intestinal epithelial cells. Am. J. Physiol. 238 (Gastrointest. Liver Physiol. 1): G190–G196, 1980.
 28. Birnbaumer, L., and B. W. O'Malley. Hormone action. I. Peptide hormones. Methods Enzymol. 109: 1–891, 1985.
 29. Bitar, K. N., S. I., Said, G. C. Weir, B. Saffouri, and G. M. Makhlouf. Neural release of vasoactive intestinal peptide from the gut. Gastroenterology 79: 1288–1294, 1980.
 30. Blay, J., and K. D. Brown. Functional receptors for epidermal growth factor in an epithelial‐cell line derived from the rat small intestine. Biochem. J. 225: 85–94, 1985.
 31. Bloom, S. R., and A. V. Edwards. Effects of autonomic stimulation on the release of vasoactive intestinal peptide from the gastrointestinal tract in the calf. J. Physiol. Lond. 299: 437–452, 1980.
 32. Bloom, S. R., J. M., Polak, and A. G. E. Pearse. Vasoactive intestinal peptide and watery diarrhea syndrome. Lancet 2: 14–16, 1973.
 33. Bloom, S. R., D. N., Ralphs, G. M. Besser, R. Hall, D. H. Coy, A. J. Kastin, and A. V. Schally. Effect of somatostatin on motilin levels and gastric emptying. Gut 16: 834–837, 1975.
 34. Boden, G., M., Sivitz, O. Owen, N. Essa‐Koumar, and J. H. Landor. Somatostatin suppresses secretin and pancreatic exocrine secretion. Science Wash. DC 190: 163–165, 1975.
 35. Boige, N., B., Amiranoff, A. Munck, and M. Laburthe. Forskolin stimulates adenylate cyclase in human colonic crypts: interaction with VIP. Eur. J. Pharmacol. 101: 111–117, 1984.
 36. Boige, N., A., Munck, and M. Laburthe. Adrenergic versus VIPergic control of cyclic AMP in human colonic crypts. Peptides Fayetteville 5: 379–383, 1984.
 37. Boissard, C., G., Hejblum, J. C. Marie, C. Gespach, and G. Rosselin. Desensibilisation au VIP par rétro‐régulation des récepteurs de ce peptide dans les cellules transformées HT‐29 de l'épithélium colique humain en culture. C. R. Hebd. Seances Acad. Sci. 299: 795–799, 1984.
 38. Bolton, J. E., and M. Field. Ca ionophore‐stimulated ion secretion in rabbit ileal mucosa: relation to actions of cyclic AMP and carbamylcholine. J. Membr. Biol. 35: 159–174, 1977.
 39. Böttcher, G., K., Sjölund, E. Ekblad, R. Håkanson, T. W. Schwartz, and F. Sundler. Coexistence of peptide YY and glicentin immunoreactivity in endocrine cells of the gut. Regul. Pept. 8: 261–266, 1984.
 40. Bouscarel, B., C., Cortinovis, C. Carpene, J. C. Murat, and H. Paris. Alpha 2‐adrenoreceptors in the HT‐29 human colon adenocarcinoma cell line: characterization with [3H]clonidine; effects on cyclic AMP accumulation. Eur. J. Pharmacol. 107: 223–231, 1985.
 41. Bower, J. M., R., Camble, H. Gregory, E. L. Gerring, and I. R. Willshire. The inhibition of gastric acid secretion by epidermal growth factor. Experientia Basel 31: 825–826, 1975.
 42. Brazeau, P., W., Vale, R. Burgus, N. Ling, M. Butcher, J. Rivier, and R. Guillemin. Hypothalamic polypeptide that inhibits the secretion of immunoreactive pituitary growth hormone. Science Wash. DC 179: 77–79, 1973.
 43. Breant, B., M. C., Chamblier, and G. Rosselin. The effect of experimental diabetes and insulin replacement on protein synthesis and RNA translation in rat enterocytes. C. R. Hebd. Seances Acad. Sci. 299: 571–574, 1984.
 44. Bromer, W. W., L. G., Sinn, A. Staub, and O. K. Behrens. The aminoacid sequence of glucagon. J. Am. Chem. Soc. 78: 3858–3860, 1956.
 45. Brown, J. C., and A. Dryburgh, JR. Gastric inhibitory polypeptide. II. The complete amino acid sequence. Can. J. Biochem. 49: 867–872, 1971.
 46. Brown, P. D., and F. V. Sepulveda. A rabbit jejunal isolated enterocyte preparation suitable for transport studies. J. Physiol. Lond. 363: 257–270, 1985.
 47. Browning, J. G., J., Hardcastle, P. T. Hardcastle, and P. A. Sanford. The role of acetylcholine in the regulation of ion transport by rat colon mucosa. J. Physiol. Lond. 272: 737–754, 1977.
 48. Broyart, J. P., C., Dupont, M. Laburthe, and G. Rosselin. Characterization of vasoactive intestinal peptide receptors in human colonic epithelial cells. J. Clin. Endocrinol. Metab. 52: 715–721, 1981.
 49. Buts, J.‐P., R. De Meyer, and J. Kolanowski. Ontogeny of cell proliferation and DNA synthesis in rat colon: role of glucocorticoids. Am. J. Physiol. 244 (Gastrointest. Liver Physiol. 7): G469–G474, 1983.
 50. Byyny, R. L., D. N., Orth, S. Cohen, and E. S. Doyne. Epidermal growth factor: effects of androgens and adrenergic agents. Endocrinology 95: 776–782, 1974.
 51. Carpene, C., H., Paris, C. Cortinovis, V. Viallard, and J. C. Murat. Characterization of alpha 2‐adrenergic receptors in the human colon adenocarcinoma cell line HT‐29 in culture by [3H]yohimbine binding. Gen. Pharmacol. 14: 701–703, 1983.
 52. Carpenter, G., and S. Cohen. Epidermal growth factor. Annu. Rev. Biochem. 48: 193–216, 1979.
 53. Carpenter, G., K. J., Lembach, M. M. Morrison, and S. Cohen. Characterization of the binding of 125I‐labeled epidermal growth factor to human fibroblasts. J. Biol. Chem. 250: 4297–4304, 1975.
 54. Carraway, R., and S. E. Leeman. The isolation of a new hypotensive peptide, neurotensin, from bovine hypothalami. J. Biol. Chem. 248: 6854–6861, 1973.
 55. Carraway, R., and S. E. Leeman. Characterization of radio‐immunoassayable neurotensin in the rat. J. Biol. Chem. 251: 7045–7052, 1976.
 56. Carter, J. H., H., Carter, J. Nussbaum, and A. Eichholz. Isolation of hamster intestinal epithelial cells using hypoosmotic media and PVP. J. Cell. Physiol. 111: 55–67, 1982.
 57. Carter, R. F., K. N., Bitar, A. M. Zfass, and G. M. Makhloup. Inhibition of VIP‐stimulated intestinal secretion and cyclic AMP production by somatostatin in the rat. Gastroenterology 74: 726–730, 1978.
 58. Cassuto, J., J., Fahrenkrug, M. Jodal, R. Tuttle, and O. Lundgren. Release of vasoactive intestinal polypeptide from the cat small intestine exposed to cholera toxin. Gut 22: 958–963, 1981.
 59. Catt, K. J., and M. L. Dufau. Peptide hormone receptors. Annu. Rev. Physiol. 39: 529–557, 1977.
 60. Catt, K. J., and M. L. Dufau. Hormone action: control of target‐cell function by peptide, thyroid, and steroid hormones. In: Endocrinology and Metabolism, edited by P. Felig, J. D. Baxter, A. E. Broadus, and L. A. Frohman. New York: McGraw‐Hill, 1981, p. 61–105.
 61. Cezard, J. P., M. E., Forgue‐Lafitte, M. C. Chamblier, and G. E. Rosselin. Growth‐promoting effect, biological activity and binding of insulin in human intestinal cancer cells in culture. Cancer Res. 41: 1148–1153, 1981.
 62. Chabot, J. G., J., Guy, R. St. Arnaud, P. Walker, and G. Pelletier. Autoradiographic localization of epidermal growth factor (EGF) receptors in different tissues of the rat (Abstract). J. Cell Biol. 95: 179a, 1982.
 63. Chabot, J. G., and J. S. Hugon. Stimulation of DNA synthesis in mouse intestinal mucosa by epidermal growth factor (EGF) (Abstract). J. Cell Biol. 87: 155a, 1980.
 64. Chang, E. B., M., Field, and R. J. Miller. Enterocyte α2‐adrenergic receptors: yohimbine and p‐aminoclonidine binding relative to ion transport. Am. J. Physiol. 244 (Gastrointest. Liver Physiol. 7): G76–G82, 1983.
 65. Chastre, E., S., Emami, G. Rosselin, and C. Gespach. Vasoactive intestinal peptide receptor activity during enterocyte‐like differentiation and retrodifferentiation of the human colonic cancerous subclone HT‐29–18. FEBS Lett. 188: 197–204, 1985.
 66. Cheng, K., and J. Larner. Intracellular mediators of insulin action. Annu. Rev. Physiol. 47: 405–424, 1985.
 67. Cohen, P. The role of protein phosphorylation in the hormonal control of enzyme activity. Eur. J. Biochem. 151: 439–448, 1985.
 68. Cohen, S. Isolation of a mouse submaxillary gland protein accelerating incisor eruption and eyelid opening in the newborn animal. J. Biol. Chem. 237: 1555–1561, 1962.
 69. Cohen, S., and G. Carpenter. Human epidermal growth factor: isolation and chemical and biological properties. Proc. Natl. Acad. Sci. USA 72: 1317–1321, 1975.
 70. Costa, M. R., J. E., Casnellie, and W. A. Catterall. Selective phosphorylation of the alpha subunit of the sodium channel by cAMP‐dependent protein kinase. J. Biol. Chem. 257: 7918–7921, 1982.
 71. Cotterell, D. J., K. A., Munday, and J. A. Poat. The binding of [3H]prazosin and [3H]clonidine to rat jejunal epithelial cell membranes. Biochem. Pharmacol. 33: 751–756, 1984.
 72. Coupar, I. M. Characterization of the opiate receptor population mediating inhibition of VIP‐induced secretion from the small intestine of the rat. Br. J. Pharmacol. 80: 371–376, 1983.
 73. Couvineau, A., S., Gammeltoft, and M. Laburthe. Molecular characteristics and peptide specificity of VIP receptors from rat cerebral cortex. J. Neurochem. 47: 1469–1475, 1986.
 74. Couvineau, A., and M. Laburthe. The human vasoactive intestinal peptide receptor: molecular identification by covalent cross‐linking in colonic epithelium. J. Clin. Endocrinol. Metab. 61: 50–55, 1985.
 75. Couvineau, A., and M. Laburthe. The rat liver vasoactive intestinal peptide binding sites. Molecular characterization by covalent cross‐linking and evidence for differences from the intestinal receptor. Biochem. J. 225: 473–479, 1985.
 76. Couvineau, A., M., Rousset, and M. Laburthe. Molecular identification and structural requirement of vasoactive intestinal peptide (VIP) receptors in the human colon adenocarcinoma cell line, HT‐29. Biochem. J. 231: 139–143, 1985.
 77. Couvineau, A., C., Rouyer‐Fessard, A. Fournier, S. St. Pierre, R. Pipkorn, and M. Laburthe. Structural requirements for VIP interaction with specific receptors in human and rat intestinal membranes: effect of nine partial sequences. Biochem. Biophys. Res. Commun. 121: 493–498, 1984.
 78. Craven, P. A., and F. R. Derubertis. Cyclic nucleotide metabolism in rat colonic epithelial cells with different proliferative activities. Bioehim. Biophys. Acta 676: 155–169, 1981.
 79. Creutzfeldt, W., and R. Ebert. Release of gastric inhibitory polypeptide (GIP) to a test meal under normal and pathological conditions in man. In: Proceed. 9th Congr. Int. Diabetes Fed. New Delhi, edited by J. S. Basaj. Amsterdam: Excerpta Medica, 1977, p. 63–75.
 80. Czernik, A. J., and B. Petrack. Somatostatin receptor binding in rat cerebral cortex. Characterization using a nonreducible somatostatin analog. J. Biol. Chem. 258: 5525–5530, 1983.
 81. Das, M., T., Miyakawa, C. F. Fox, R. M. Pruss, A. Aharonov, and H. R. Herschman. Specific radiolabeling of a cell surface receptor for epidermal growth factor. Proc. Natl. Acad. Sci. USA 74: 2790–2794, 1977.
 82. Dashwood, M. R., E. S., Debnam, J. Bagnall, and C. S. Thompson. Autoratiographic localization of opiate receptors in rat small intestine. Eur. J. Pharmacol. 107: 267–269, 1985.
 83. Davis, G. R., C. A. Santa, Ana, S. G. Morawski, and J. S. Fordtran. Effect of vasoactive intestinal polypeptide on active and passive transport in the human jejunum. J. Clin. Invest. 67: 1687–1694, 1981.
 84. De Jonge, H. R. Cyclic nucleotide‐dependent phosphorylation of intestinal epithelium proteins. Nature Lond. 262: 590–593, 1976.
 85. De Jonge, H. R., and F. S. Van Dommelen. Cyclic GMP‐dependent phosphorylation and ion transport in intestinal microvilli. In: Protein Phosphorylation, edited by O. M. Rosen and E. G. Krebs. Cold Spring Harbor, NY: Cold Spring Harbor, 1981, vol. 8, p. 1313–1332. (Cold Spring Harbor Conf. Cell Proliferation.)
 86. De Rubertis, F. R., and P. A. Craven. Early alterations in rat colonic mucosal cyclic nucleotide metabolism and protein kinase activity induced by 1,2‐dimethylhydrazine. Cancer Res. 40: 4589–4598, 1980.
 87. Dharmsathaphorn, K., H., Binder, and J. W. Dobbins. Somatostatin stimulates sodium and chloride absorption in the rabbit ileum. Gastroenterology 78: 1559–1565, 1980.
 88. Dharmsathaphorn, K., V., Harms, D. J. Yamashiro, R. J. Hughes, H. J. Binder, and E. M. Wright. Preferential binding of vasoactive intestinal polypeptide to basolateral membrane of rat and rabbit enterocytes. J. Clin. Invest. 71: 27–35, 1983.
 89. Dharmsathaphorn, K., K. G., Mandel, H. Masui, and J. A. Mcroberts. Vasoactive intestinal polypeptide‐induced chloride secretion by a colonic epithelial cell line. Direct participation of the basolaterally localized Na+, K +, Cl‐ cotransport system. J. Clin. Invest. 75: 462–471, 1985.
 90. Dharmasathaphorn, K., J. A., Mcroberts, K. G. Mandel, L. D. Tisdale, and H. Masui. A human colonic tumor cell line that maintains vectorial electrolyte transport. Am. J. Physiol. 246 (Gastrointest. Liver Physiol. 9): G204–G208, 1984.
 91. Dharmasathaphorn, K., L., Racusen, and J. W. Dobbins. Effect of somatostatin on ion transport in the rat colon. J. Clin. Invest. 66: 813–820, 1980.
 92. Diani, A. R., G. C., Gerritsen, S. Stromsta, and P. Murray. A study of morphological changes in the small intestine of the spontaneously diabetic Chinese hamster. Diabetologia 12: 101–109, 1976.
 93. Dobbins, J., L., Racusen, and H. J. Binder. Effect of D‐alanine methionine enkephalin amide on ion transport in rabbit ileum. J. Clin. Invest. 66: 19–28, 1980.
 94. Donowitz, M., M. E., Cohen, R. Gudewich, L. Taylor, and G. W. G. Sharp. Ca2+‐calmodulin‐, cyclic AMP‐, and cyclic GMP‐induced phosphorylation of proteins in purified microvillus membranes of rabbit ileum. Biochem. J. 219: 573–581, 1984.
 95. Donowitz, M., G., Elta, L. Battisti, R. Fogel, and E. Schwartz. Effect of dopamine and bromocriptine on rat ileal and colonic transport, stimulation of absorption and reversal of cholera‐induced secretion. Gastroenterology 84: 516–523, 1983.
 96. Donowitz, M., S., Levin, G. Powers, G. Elta, P. Cohen, and H. Cheng. Ca2+ channel blockers stimulate ileal and colonic water absorption. Gastroenterology 89: 858–866, 1985.
 97. Dupont, C., B., Amiranoff, M. Laburthe, and G. Rosselin. Récepteurs du peptide intestinal vasoactif (VIP) dans les membranes d'adénocarcinome colique humain: liaison spécifique et stimulation de l'adénylate cyclase. C. R. Hebd. Seances Acad. Sci. 286: 209–212, 1978.
 98. Dupont, C., M., Laburthe, J. P. Broyart, D. Bataille, and G. Rosselin. Cyclic AMP production in isolated colonic epithelial crypts: a highly sensitive model for the evaluation of vasoactive intestinal peptide action in human intestine. Eur. J. Clin. Invest. 10: 67–76, 1980.
 99. Ebeid, A. M., P. B., Soeters, P. Murray, and J. E. Fischer. Release of vasoactive intestinal peptide (VIP) by intraluminal osmotic stimuli. J. Surg. Res. 23: 25–30, 1977.
 100. Edmonds, C. J., and J. Marriott. Sodium transport and short circuit current in rat colon in vivo and the effect of aldosterone. J. Physiol. Lond. 210: 1021–1039, 1970.
 101. Edwards, A. V., P. M. M., Bricham, S. J. Mitchell, and S. R. Bloom. Changes in the concentration of vasoactive intestinal peptide in the intestinal lymph in response to vagal stimulation in the calf. Experientia Basel 34: 1186–1187, 1978.
 102. Ekblad, E., R., Håkanson, and F. Sundler. VIP and PHI coexist with an NPY‐like peptide in intramural neurones of the small intestine. Regul. Pept. 10: 47–55, 1984.
 103. Eklund, S., M., Jodal, O. Lundgren, and A. Sjoqvist. Effects of vasoactive intestinal polypeptide on blood flow, motility and fluid transport in the gastrointestinal tract of the cat. Acta Physiol. Scand. 105: 461–468, 1979.
 104. El Battari, A., E. Ah‐Hye, J. M. Muller, H. Sari, and J. Marvaldi. Modification of HT‐29 cell response to the vasoactive intestinal peptide (VIP) by membrane fluidization (Abstract). Regul. Pept. Suppl. 3: S38, 1985.
 105. Elder, J. B., G., Williams, B. Lacy, and H. Gregory. Cellular localization of human urogastrone/epidermal growth factor. Nature Lond. 271: 466–467, 1978.
 106. El‐Salhy, M., and L. Grimelius. Immunocytochemical demonstration of polypeptide YY (PYY) in the gastrointestinal tract of the monkey, Macaca rhesus: a light and electron microscopy study. Biomed. Res. 4: 289–294, 1983.
 107. El‐Salhy, M., L., Grimelius, E. Wilander, B. Ryberg, L. Terenius, J. M. Lundberg, and K. Tatemoto. Immunocytochemical identification of polypeptide YY (PYY) cells in the human gastrointestinal tract. Histochemistry 77: 15–23, 1983.
 108. El‐Salhy, M., E., Wilander, L. Grimelius, L. Terenius, J. M. Lundberg, and K. Tatemoto. The distribution of polypeptide YY (PYY)‐ and pancreatic polypeptide (PP)‐immunoreactive cells in the domestic fowl. Histochemistry 75: 25–30, 1982.
 109. El‐Salhy, M., E., Wilander, L. Juntti‐Berggren, and L. Grimelius. The distribution and ontogeny of polypeptide YY (PYY)‐ and pancreatic polypeptide (PP)‐immunoreactive cells in the gastrointestinal tract of rat. Histochemistry 78: 53–60, 1983.
 110. Emson, P. C., and M. E. Dequidt. NPY: a new member of the pancreatic polypeptide family. Trends Neurosci. 7: 31–35, 1984.
 111. Eng, J., Y. C. E., Pan, J. P. Raufman, and R. S. Yalow. Purification and sequencing of dog and guinea pig VIP's (Abstract). Regul. Pept. Suppl. 3: S14, 1985.
 112. Enjalbert, A., L., Tapia‐Arancibia, M. Rieutort, P. Brazeau, C. Kordon, and J. Epelbaum. Somatostatin receptors on rat anterior pituitary membranes. Endocrinology 110: 1634–1640, 1982.
 113. Evans, E. M., J. M., Wrigglesworth, K. Burdett, and W. F. R. Pover. Studies on epithelial cells isolated from guinea pig small intestine. J. Cell Biol. 51: 452–464, 1971.
 114. Fahrenkrug, J., H., Galbo, J. J. Holst, and O. B. Schaffalitzky Demuckadell. Influence of the autonomic nervous system on the release of vasoactive intestinal polypeptide from the porcine gastrointestinal tract. J. Physiol. Lond. 280: 405–422, 1978.
 115. Fahrenkrug, J., V., Haglund, M. Jodal, O. Lundgren, L. Olbe, and O. B. Schaffalitzky Demuckadell. Nervous release of vasoactive intestinal polypeptide in the gastrointestinal tract of cats: possible physiological implications. J. Physiol. Lond. 284: 291–305, 1978.
 116. Feldman, E. J., D., Aures, and M. I. Grossman. Epidermal growth factor stimulates ornithine decarboxylase activity in the digestive tract of mouse. Proc. Soc. Exp. Biol. Med. 159: 400–402, 1978.
 117. Field, M. Cholera toxin, adenylate cyclase and the process of active secretion in the small intestine: the pathogenesis of diarrhea in cholera. In: The Physiological Basis for Disorders of Biomembranes, edited by T. E. Andreoli, J. F. Hoffman, and D. D. Fanestil. New York: Plenum, 1978, vol. 5, p. 877–899.
 118. Field, M. Intracellular mediators of secretion in the small intestine. In: Mechanisms of Intestinal Secretion, edited by H. J. Binder. New York: Liss, 1979, p. 83–91. (Kroc Found. Ser. 12.)
 119. Field, M. Secretion of electrolytes and water by mammalian small intestine. In: Physiology of the Gastrointestinal Tract (1st ed.), edited by L. R. Johnson. New York: Raven, 1981, p. 963–982.
 120. Field, M., H. E., Sheerin, A. Henderson, and P. L. Smith. Catecholamine effects on cyclic AMP levels and ion secretion in rabbit ileal mucosa. Am. J. Physiol. 229: 86–92, 1975.
 121. Fogel, R., and R. B. Kaplan. Role of enkephalins in regulation of basal intestinal water and ion absorption in the rat. Am. J. Physiol. 246 (Gastrointest. Liver Physiol. 9): G386–G392, 1984.
 122. Forgue‐Lafitte, M. E., A., Horvat, and G. Rosselin. Insulin binding by a cell line (HT‐29) derived from human colonic cancer. Mol. Cell. Endocr. 14: 123–130, 1979.
 123. Forgue‐Lafitte, M. E., M., Laburthe, M. C. Chamblier, A. J. Moody, and G. Rosselin. Demonstration of specific receptors for EGF‐urogastrone in isolated rat intestinal epithelial cells. FEBS Lett. 114: 243–246, 1980.
 124. Forgue‐Lafitte, M. E., M. R., Marescot, M. C. Chamblier, and G. Rosselin. Evidence for the presence of insulin binding sites in isolated rat intestinal epithelial cells. Diabetologia 19: 373–378, 1980.
 125. Fox, C. F., P. S., Linsley, and M. Wrann. Receptor remodeling and regulation in the action of epidermal growth factor. Federation Proc. 11: 2988–2995, 1982.
 126. Frizzell, R. A. Active chloride secretion by rabbit colon: calcium‐dependent stimulation by ionophore A 23187. J. Membr. Biol. 35: 175–187, 1977.
 127. Frizzel, R. A., and K. Heintze. Electrogenic chloride secretion by mammalian colon. In: Mechanisms of Intestinal Secretion, edited by H. J. Binder. New York: Liss, 1979, p. 101–110. (Kroc Found. Ser. 12.)
 128. Frolich, J. C., I., Reimann, R. Storz, D. Rabin, Z. T. Bloomgarden, S. R. Bloom, J. Oates, and W. A. Muller. Wirkung von Somatostatin beim Carcinoid‐und Verner‐Morrison‐Syndrom. Verh. Dtsch. Ges. Inn. Med. 85: 174–175, 1979.
 129. Furness, J. B., M., Costa, P. C. Emson, R. Håkanson, E. Moghimzadeh, F. Sundler, I. L. Taylor, and R. E. Chance. Distribution, pathways and reactions to drug treatment of nerves with neuropeptide Y‐ and pancreatic polypeptide‐like immunoreactivity in the guinea pig digestive tract. Cell Tissue Res. 234: 71–92, 1983.
 130. Furness, J. B., M., Costa, and J. R. Keast. Choline acetyl‐transferase‐ and peptide‐immunoreactivity of submucous neurons in the small intestine of the guinea pig. Cell Tissue Res. 237: 329–336, 1984.
 131. Fuxe, K., T., Hökfelt, S. I. Said, and V. Mutt. Vasoactive intestinal polypeptide and the nervous system: immuno‐histochemical evidence for localization in central and peripheral neurons, particularly intracortical neurons of the cerebral cortex. Neurosci. Lett. 5: 241–246, 1977.
 132. Gaginella, T. S. Neuroregulation of intestinal ion transport. Trends Pharmacol. Sci. 5: 397–399, 1984.
 133. Gaginella, T. S., H. S., Mekhjian, and T. M. O'Dorisio. Vasoactive intestinal peptide: quantification by radioimmunoassay in isolated cells, mucosa and muscle of the Hamster intestine. Gastroenterology 74: 11–15, 1978.
 134. Gaginella, T. S., and T. M. O'Dorisio. Vasoactive intestinal polypeptide: neuromodulator of intestinal secretion? In: Mechanisms of Intestinal Secretion, edited by H. J. Binder. New York: Liss, 1979, p. 231–247. (Kroc Found. Ser. 12.)
 135. Gaginella, T. S., T. M., O'Dorisio, and K. A. Hubel. Release of vasoactive intestinal polypeptide by electrical field stimulation of rabbit ileum. Regul. Pept. 2: 165–174, 1981.
 136. Gaginella, T. S., S. F., Phillips, R. R. Dozois, and V. L. W. Go. Stimulation of adenylate cyclase in homogenates of isolated intestinal epithelial cells from hamsters. Effects of gastrointestinal hormones, prostaglandins, and deoxycholic and ricinoleic acids. Gastroenterology 74: 11–15, 1978.
 137. Gaginella, T. S., T. J., Rimele, and M. Wietecha. Studies on rat intestinal epithelial cell receptors for serotonin and opiates. J. Physiol. Lond. 335: 101–111, 1983.
 138. Gallo‐Payet, N., and J. S. Hugon. Insulin receptors in isolated adult mouse intestinal cells: studies in vivo and in organ culture. Endocrinology 114: 1885–1892, 1984.
 139. Gallo‐Payet, N., and J. S. Hugon. Epidermal growth factor receptors in isolated adult mouse intestinal cells: studies in vivo and in organ culture. Endocrinology 116: 194–201, 1985.
 140. Gammeltoft, S. Insulin receptors: binding kinetics and structure‐function relationship of insulin. Physiol. Rev. 64: 1321–1378, 1984.
 141. Gardner, J. D. Receptors for gastrointestinal hormones. Gastroenterology 76: 202–214, 1979.
 142. Gershon, M. D., and S. M. Erde. The nervous system of the gut. Gastroenterology 80: 1571–1594, 1981.
 143. Gespach, C., E. Chastre, S. Emami, and N. Mulliez. Vasoactive intestinal peptide receptor activity in human fetal enterocytes. FEBS Lett. 180: 196–202, 1985.
 144. Gilman, A. G. Guanine nucleotide‐binding regulatory proteins and dual control of adenylate cyclase. J. Clin. Invest. 73: 1–4, 1984.
 145. Gomez, S., A., Morel, P. Nicolas, and P. Cohen. Regional distribution of the Mr 15,000 somatostatin precursor, somatostatin‐28 and somatostatin‐14 in the rat brain suggests a differential intracellular processing of the high molecular weight species. Biochem. Biophys. Res. Commun. 112: 297–305, 1983.
 146. Granger, D. N., P. R., Kvietys, W. H. Wilborn, N. A. Mortillaro, and A. E. Taylor. Mechanism of glucagon‐induced intestinal secretion. Am. J. Physiol. 239 (Gastrointest. Liver Physiol. 2): G30–G38, 1980.
 147. Grasset, E., J., Bernabeu, and M. Pinto. Epithelial properties of human colonic carcinoma cell line Caco‐2: effect of secretagogues. Am. J. Physiol. 248 (Cell Physiol. 17): C410–C418, 1985.
 148. Grasset, E., M., Pinto, E. Dussaulx, A. Zweibaum, and J.‐F. Desjeux. Epithelial properties of human colonic carcinoma cell line Caco‐2: electrical parameters. Am. J. Physiol. 247 (Cell Physiol. 16): C260–C267, 1984.
 149. Greengard, P. Phosphorylated proteins as physiological effectors. Science Wash. DC 199: 146–152, 1978.
 150. Grube, D., and W. G. Forssmann. Morphology and function of the entero‐endocrine cells. Horm. Metab. Res. 11: 589–646, 1979.
 151. Guandalini, S., J. F., Kachur, P. L. Smith, R. J. Miller, and M. Field. In vitro effects of somatostatin on ion transport in rabbit intestine. Am. J. Physiol. 238 (Gastrointest. Liver Physiol. 1): G67–G74, 1980.
 152. Guillemin, R., P., Brazeau, P. Bohlen, F. Esch, N. Ling, and W. R. Wehrenberg. Growth hormone‐releasing factor from a human pancreatic tumor that caused acromegaly. Science Wash. DC 218: 585–587, 1982.
 153. Guillemin, R., and J. Gerich. Somatostatin: physiological and clinical significance. Annu. Rev. Med. 27: 379–388, 1978.
 154. Haffen, K., M. J. H., Lewin, and P. Robberecht. Intérět du moděle des cellules isolées du pancréas exocrine, de l'estomac et de l'intestin grěle pour la recherche en gastroentérologie. Gastroenterol. Clin. Biol. 3: 267–282, 1979.
 155. Hardcastle, J., P. T., Hardcastle, and J. M. Noble. The involvement of calcium in the intestinal response to secretagogues in the rat. J. Physiol. Lond. 355: 465–478, 1984.
 156. Harrer, D. S., B. K., Stern, and R. W. Reylly. Removal and dissociation of epithelial cells from the rodent gastrointestinal tract. Nature Lond. 203: 319–320, 1964.
 157. Harrison, D. D., and H. L. Webster. The preparation of isolated intestinal crypt cells. Exp. Cell Res. 55: 257–260, 1969.
 158. Hauri, H. P., E. E., Sterchi, D. Bienz, J. A. M. Fransen, and A. Marxer. Expression and intracellular transport of microvillus membrane hydrolases in human intestinal epithelial cells. J. Cell Biol. 101: 838–851, 1985.
 159. Helmstaedter, V., G. E., Feurle, and W. G. Forssmann. Ultrastructural identification of a new cell type: the N‐cell as the source of neurotensin in the gut mucosa. Cell Tissue Res. 184: 445–452, 1977.
 160. Hengl, G., J., Prager, and H. Pointner. The influence of somatostatin on the absorption of triglycerides in partially gastrectomized subjects. Acta Hepato‐Gastroenterol. 26: 392–395, 1979.
 161. Henning, S. J. Ontogeny of enzymes in the small intestine. Annu. Rev. Physiol. 47: 231–245, 1985.
 162. Hirata, Y., and O. N. Orth. Epidermal growth factor (urogastrone) in human fluids. Size heterogeneity. J. Clin Endocrinol. Metab. 48: 673–679, 1979.
 163. Hökfelt, T., O., Johansson, S. Effendic, R. Luft, and A. Arimura. Are there somatostatin‐containing nerves in the rat gut? Immunohistochemical evidence for a new type of peripheral nerves. Experientia Basel 31: 852–854, 1975.
 164. Hollenberg, M. D., and P. Cuatrecasas. Insulin and epidermal growth factor. Human fibroblast receptors related to desoxyribonucleic acid synthesis and aminoacid uptake. J. Biol. Chem. 250: 3845–3853, 1975.
 165. Hopfer, U. Diabetes mellitus: changes in the transport properties of isolated intestinal microvillous membranes. Proc. Natl. Acad. Sci. USA. 72: 2027–2031, 1975.
 166. Hoshino, M., C., Yanaihara, Y. M. Hong, S. Kishida, Y. Katsumaru, A. Vandermeers, M. C. Vandermeers‐Piret, P. Robberecht, J. Christophe, and N. Yanaihara. Primary structure of helodermin, a VIP‐secretin‐like peptide isolated from Gila monster venom. FEBS Lett. 178: 233–239, 1984.
 167. Huang, K. C. Uptake of L‐tyrosine and 3,o‐methylglucose by isolated intestinal epithelial cells. Life Sci. 4: 1201–1206, 1965.
 168. Hubel, K. A. Intestinal nerves and ion transport: stimuli, reflexes, and responses. Am. J. Physiol. 248 (Gastrointest. Liver Physiol. 11): G261–G271, 1985.
 169. Hubel, K. A., T. S., Gaginella, and Tm., O'Dorisio. Release of vasoactive intestinal peptide by electrical field stimulation of rabbit ileum (Abstract). Gastroenterology 74: 1127, 1978.
 170. Hughes, J. Isolation of an endogenous compound from the brain with pharmacological properties similar to morphine. Brain Res. 88: 295–308, 1975.
 171. Hughes, J., T. W., Smith, H. W. Kosterlitz, L. A. Fothergill, B. A. Morgan, and H. R. Morris. Identification of two related pentapeptides from the brain with potent opiate agonist activity. Nature Lond. 253: 577–579, 1975.
 172. Hunt, L. T., and M. O. Dayhoff. Hormones and active peptides. In: Atlas of Protein Sequence and Structure, edited by M. O. Dayhoff. Silver Spring, MD: National Biomedical Research Foundation, 1976, vol. 5, suppl. II, p. 113–145.
 173. Hyun, C. S., E. J., Cragoe, JR, and M. Field. α2‐Adrenergic receptor‐mediated regulation of intestinal calcium transport. Am. J. Physiol. 249 (Cell Physiol. 18): C117–C123, 1985.
 174. Ilundain, A., and R. J. Naftalin. Role of Ca2+‐dependent regulator protein in intestinal secretion. Nature Lond. 279: 446–448, 1979.
 175. Isenberg, J. I., B., Wallin, C. Johansson, B. Smedfors, V. Mutt, K. Tatemoto, and S. Emas. Secretin, VIP and PHI stimulate rat proximal duodenal surface epithelial bicarbonate secretion in vivo. Regul. Pept. 8: 315–320, 1984.
 176. Itoh, N., K., Obata, N. Yanaihara, and H. Okamoto. Human prepro‐vasoactive intestinal polypeptide (VTP) mRNA contains the coding sequence for a novel PHI‐27 like peptide, PHM‐27. Nature Lond. 304: 547–549, 1983.
 177. Jessen, K. R., M. J., Saffrey, S. Van Noorden, S. R. Bloom, J. M. Polak, and G. Burnstock. Immunohistochemical studies of the enteric nervous system in tissue culture and in situ: localization of vasoactive intestinal peptide (VIP), sub‐stance‐P and enkephalin immunoreactive nerves in the guinea pig gut. Neuroscience 5: 1717–1735, 1980.
 178. Johnson, L. R. Effect of gastrointestinal hormones on growth of gastrointestinal tissue. In: Gastrointestinal Hormones, edited by G. B. Jerzy Glass. New York: Raven, 1980, p. 507–527.
 179. Johnson, L. R., and P. D. Guthrie. Stimulation of rat oxyntic gland mucosa growth by epidermal growth factor. Am. J. Physiol. 238 (Gastrointest. Liver Physiol. 1): G45–G49, 1980.
 180. Kachur, J. R., and R. J. Miller. Characterization of the opiate receptor in the guinea pig ileal mucosa. Eur. J. Pharmacol. 81: 177–183, 1982.
 181. Kachur, J. F., R. J., Miller, and M. Field. Control of guinea pig intestinal electrolyte secretion by a delta‐opiate receptor. Proc. Natl. Acad. Sci. USA 77: 2753–2756, 1980.
 182. Kachur, J. F., R. J., Miller, M. Field, and J. Rivier. Neurohumoral control of ileal electrolyte transport. II. Neurotensin and substance P. J. Pharmacol. Exp. Ther. 220: 456–463, 1982.
 183. Kahn, C. R. Membrane receptors for hormones and neurotransmitters. J. Cell Biol. 70: 261–286, 1976.
 184. Kahn, C. R., K. L., Baird, J. S. Flier, C. Grunfeld, J. T. Harmon, L. C. Harrison, F. A. Karlsson, M. Kasuga, G. L. King, U. C. Lang, J. M. Podskalny, and E. Van Obberghen. Insulin receptors, receptor antibodies, and the mechanism of insulin action. Recent Prog. Horm. Res. 37: 447–538, 1981.
 185. Keast, J. R., J. B., Furness, and M. Costa. Origin of peptide and norepinephrine nerves in the mucosa of the guinea pig small intestine. Gastroenterology 86: 637–644, 1984.
 186. Kedinger, M., P. M., Simon‐Assmann, B. Lacroix, and K. Haffen. Role of glucocorticoids on the maturation of brush border enzymes in fetal rat gut endoderm. Experientia Basel 39: 1150–1152, 1983.
 187. Kellett, G. L., A., Jamal, J. P. Robertson, and N. Wollen. The acute regulation of glucose absorption, transport and metabolism in rat small intestine by insulin in vivo. Biochem. J. 219: 1027–1035, 1984.
 188. Kimberg, D. V., L. J., Shlatz, and K. A. Cattieu. Cyclic nucleotide‐dependent protein kinases in membranes from rat small intestine. In: Mechanisms of Intestinal Secretion, edited by H. J. Binder. New York: Liss, 1979, p. 131–146. (Kroc Found. Ser. 12.)
 189. Kimmel, J. R., L. J., Hayden, and H. G. Pollock. Isolation and characterization of a new pancreatic polypeptide hormone. J. Biol. Chem. 250: 9639–9645, 1975.
 190. Kimmel, J. R., H. G., Pollock, R. E. Chance, M. G. Johnson, J. R. Reeve, I. L. Taylor, C. Miller, and J. E. Shively. Pancreatic polypeptide from rat pancreas. Endocrinology 114: 1725–1731, 1984.
 191. Kimmich, G. A. Preparation and properties of mucosal epithelial cells from small intestine of the chicken. Biochemistry 9: 3659–3668, 1970.
 192. Kimmich, G. A. Preparation and characterization of isolated intestinal cells and their use in studying intestinal transport. In: Methods in Membrane Biology, edited by E. Korn. New York: Plenum, 1975, vol. 4, p. 51–115.
 193. Kirkegaard, P., J. M., Lundberg, S. S. Poulsen, P. S. Olsen, J. Fahrenkrug, T. Hökfelt, and J. Christiansen. Vasoactive intestinal polypeptidergic nerves and Brunner's gland secretion in the rat. Gastroenterology 81: 872–878, 1981.
 194. Kirkegaard, P., P. S., Olsen, E. Nexo, J. J. Holst, and S. S. Poulsen. Effect of vasoactive intestinal polypeptide and somatostatin on secretion of epidermal growth factor and bicarbonate from Brunner's gland. Gut 25: 1225–1229, 1984.
 195. Kirkegaard, P., P. S., Olsen, S. S. Poulsen, and E. Nexo. Exocrine secretion of epidermal growth factor from Brunner's glands, stimulation by VIP and acetylcholine. Regul. Pept. 7: 367–372, 1983.
 196. Kirkland, S. C. Dome formation by a human colonic adenocarcinoma cell line (HCA‐7). Cancer Res. 43: 3790–3795, 1985.
 197. Kitabgi, P., R. Carra, Way, and S. E. Leeman. Isolation of a tridecapeptide from bovine intestinal tissue and its partial characterization of neurotensin. J. Biol. Chem. 251: 7053–7058, 1976.
 198. Kitabgi, P., R., Carraway, J. Van Rietshoten, C. Granier, J. L. Morgat, A. Menez, S. Leeman, and P. Freychet. Neurotensin: specific binding to synaptic membranes from rat brain. Proc. Natl. Acad. Sci. USA 74: 1846–1850, 1977.
 199. Kitabgi, P., and P. Freychet. Neurotensin contracts the guinea pig longitudinal ileal smooth muscle by inducing acetylcholine release. Eur. J. Pharmacol. 56: 403–406, 1979.
 200. Kitabgi, P., C., Poustis, C. Granier, J. Van Rietschoten, J. Rivier, J. L. Morgat, and P. Freychet. Neurotensin binding to extraneural and neural receptors: comparison with biological activity and structure‐activity relationships Mol. Pharmacol. 18: 11–19, 1980.
 201. Kitabgi, P., C., Poustis, A. Zweibaum, and P. Freychet. Peptide receptors in colonic tumor cells: specific binding of epidermal growth factor to the HT‐29 cell line. In: Hormone Receptors in Digestion and Nutrition, edited by G. Rosselin, P. Fromageot, and S. Bonfils. Amsterdam: Elsevier/North‐Holland, 1979, p. 255–260.
 202. Klaevman, H. L., T. P., Conlon, A. G. Levy, and J. D. Gardner. Effects of gastrointestinal hormones on adenylate cyclase activity in human jejunal mucosa. Gastroenterology 68: 667–675, 1975.
 203. Konturek, S. J. Somatostatin and opiate peptides: their action on gastrointestinal secretions. In: Gastrointestinal Hormones, edited by G. B. Jerzy Glass. New York: Raven, 1980, p. 693–716.
 204. Krejs, G. J., R. M., Barkley, N. W. Read, and J. S. Fordtran. Intestinal secretion induced by vasoactive intestinal polypeptide. A comparison with cholera toxin in the canine jejunum in vivo. J. Clin. Invest. 61: 1337–1345, 1978.
 205. Krejs, G. J., R., Browne, and P. Raskin. Effect of intravenous somatostatin on jejunal absorption of glucose, amino acids, water, and electrolytes. Gastroenterology 78: 26–31, 1980.
 206. Krejs, G. J., and J. S. Fordtran. Effect of VIP infusion on water and ion transport in the human jejunum. Gastroenterology 78: 722–727, 1980.
 207. Krulich, L., A. P. S., Dhariwal, and S. M. Mccann. Stimulatory and inhibitory effects of purified hypothalamic extracts on growth hormone release from rat pituitary in vitro. Endocrinology 83: 783–790, 1968.
 208. Laburthe, M., B., Amiranoff, N. Boige, C. Rouyer‐Fessard, K. Tatemoto, and L. Moroder. Interaction of GRF with VIP receptors and stimulation of adenylate cyclase in rat and human intestinal epithelial membranes. Comparison with PHI and secretin. FEBS Lett. 159: 89–92, 1983.
 209. Laburthe, M., B., Amiranoff, and C. Boissard. α‐Adrenergic inhibition of cyclic AMP accumulation in epithelial cells isolated from rat small intestine. Biochim. Biophys. Acta 721: 101–108, 1982.
 210. Laburthe, M., D., Bataille, and G. Rosselin. Vasoactive intestinal peptide (VIP): variation of the jejuno‐ileal content in the developing rat as measured by radioreceptorassay. Acta Endocrinol. 84: 588–599, 1977.
 211. Laburthe, M., D., Bataille, M. Rousset, J. Besson, Y. Broer, A. Zweibaum, and G. Rosselin. The expression of cell surface receptors for VIP, secretin and glucagon in normal and malignant cells of the digestive tract. In: Membrane Proteins, edited by P. Nicholls, J. V. Moller, P. Leth, and A. J. Moody. New York: Pergamon, 1978, vol. 45, p. 271–290.
 212. Laburthe, M., J., Besson, D. Hui Bon Hoa, and G. Rosselin. Récepteurs du peptide intestinal vasoactif (VIP) dans les enterocytes: liaison specifique et stimulation de l'AMP cyclique. C. R. Hebd. Seances Acad. Sci. 284: 2139–2142, 1977.
 213. Laburthe, M., B., Breant, and C. Rouyer‐Fessard. Molecular identification of VIP receptors in rat intestinal epithelium by covalent cross‐linking: evidence for two classes of binding sites with different structural and functional properties. Eur. J. Biochem. 139: 181–187, 1984.
 214. Laburthe, M., B., Chenut, C. Rouyer‐Fessard, K. Tatemoto, A. Couvineau, A. Servin, and B. Amiranoff. Interaction of peptide YY with rat intestinal epithelial plasma membranes: binding of the radioiodinated peptide. Endocrinology 118: 1910–1917, 1986.
 215. Laburthe, M., A., Couvineau, M. Rousset, and C. Rouyer‐Fessard. Cellular expression and molecular identification of peripheral VIP receptors (Abstract). Regul. Pept. Suppl. 3: S4, 1985.
 216. Laburthe, M., A., Couvineau, and C. Rouyer‐Fessard. Study of species specificity in growth hormone releasing factor (GRF) interaction with vasoactive intestinal peptide (VIP) receptors using GRF and intestinal VIP receptors from rat and human: evidence that Ac‐Tyr1hGRF is a competitive VIP antagonist in the rat. Mol. Pharmacol. 29: 23–27, 1986.
 217. Laburthe, M., A., Couvineau, C. Rouyer‐Fessard, and L. Moroder. Interaction of PHM, PHI and 24‐glutamine PHI with human VIP receptors from colonic epithelium: comparison with rat intestinal receptors. Life Sci. 36: 991–995, 1985.
 218. Laburthe, M., and C. Dupont. VIPergic control of intestinal epithelium in health and disease. In: Advances in Peptide Hormone Research Series, edited by S. I. Said. New York: Raven, 1982, vol. 1, p. 407–423.
 219. Laburthe, M., C., Dupont, J. Besson, M. Rousset, and G. Rosselin. A new bioassay of VIP: results in watery diarrhea syndrome. Gut 21: 619–625, 1980.
 220. Laburthe, M., P., Mangeat, G. Marchis‐Mouren, and G. Rosselin. Activation of cyclic AMP‐dependent protein kinases by vasoactive intestinal peptide (VIP) in isolated intestinal epithelial cells from rat. Life Sci. 25: 1931–1938, 1979.
 221. Laburthe, J., J., Prieto, B. Amiranoff, C. Dupont, J. Broyart, D. Hui Bon Hoa, Y. Broer, and G. Rosselin. VIP receptors in intestinal epithelial cells. Distribution throughout the intestinal tract. In: Hormone Receptors in Digestion and Nutrition, edited by G. Rosselin, P. Fromageot, and S. Bonfils. Amsterdam: Elsevier/North‐Holland, 1979, p. 241–254.
 222. Laburthe, M., J. C., Prieto, B. Amiranoff, C. Dupont, D. Hui Bon Hoa, and G. Rosselin. Interaction of vasoactive intestinal peptide with isolated intestinal epithelial cells from rat. 2. Characterization and structural requirements of the stimulatory effect of vasoactive intestinal peptide on production of adenosine 3':5'‐monophosphate. Eur. J. Biochem. 96: 239–248, 1979.
 223. Laburthe, M., G., Rosselin, M. Rousset, A. Zweibaum, M. Korner, Z. Selinger, and M. Schramm. Transfer of the hormone receptor for vaso‐intestinal peptide to an adenylate cyclase system in another cell. FEBS Lett. 98: 237–239, 1979.
 224. Laburthe, M., M., Rousset, C. Boissard, G. Chevalier, A. Zweibaum, and G. Rosselin. Vasoactive intestinal peptide: a potent stimulator of adenosine 3':5' ‐cyclic monophosphate accumulation in gut carcinoma cell lines in culture. Proc. Natl. Acad. Sci. USA 75: 2772–2775, 1978.
 225. Labuhthe, M., M., Rousset, G. Chevalier, C. Boissard, C. Dupont, A. Zweibaum, and G. Rosselin. Vasoactive intestinal peptide control of cyclic adenosine 3':5'‐monophosphate in seven human colorectal adenocarcinoma cell lines in culture. Cancer Res. 40: 2529–2533, 1980.
 226. Laburthe, M., C., Rouyer‐Fessard, A. Couvineau, K. Tatemoto, B. Chenut, and A. Servin. PPY receptors in the intestinal epithelium: preliminary characterization of a new class of peptide receptors. In: Regulatory Peptides in Digestive, Nervous and Endocrine System, edited by M. J. M. Lewin and S. Bonfils. Amsterdam: Elsevier, 1985, p. 169–172. (INSERM Symp. No. 25.)
 227. Larsson, L. I. Ultrastructural localization of a new neuronal peptide (VIP). Histochemistry 54: 173–175, 1977.
 228. Larsson, L.‐I. Peptide secretory pathways in GI tract: cytochemical contributions to regulatory physiology of the gut. Am. J. Physiol. 239 (Gastrointest. Liver Physiol. 2): G237–G246, 1980.
 229. Larsson, L. I., J., Fahrenkrug, O. B. Schaffalitzky Demuckadell, F. Sundler, R. Håkanson, and J. F. Rehfeld. Localization of vasoactive intestinal polypeptide (VIP) to central and peripheral neurons. Proc. Natl. Acad. Sci. USA 73: 3197–3200, 1976.
 230. Larsson, L. I., J. M., Polack, R. Buffa, F. Sundler, and E. Solcia. On the immunocytochemical localization of the vasoactive intestinal polypeptide. J. Histochem. Cytochem. 27: 936–938, 1979.
 231. Larsson, L. I., F. Sundler, and R., Håkanson. Immunohistochemical localization of human pancreatic polypeptide (hPP) to a population of islet cells. Cell Tissue Res. 156: 167–171, 1975.
 232. Lazarus, L. H., M. R., Brown, and M. H. Perrin. Distribution, localization and characteristics of neurotensin binding sites in the brain. Neuropharmacology 16: 625–629, 1977.
 233. Lazo, P. S., A., Rivaya, and G. Velasco. Regulation by calcium and calmodulin of adenylate cyclase from rabbit intestinal epithelium. Biochim. Biophys. Acta 798: 361–367, 1984.
 234. Lee, D. K. H., and S. Solomon. Characteristics and ontogeny of nuclear receptor for glucocorticoids in the rabbit fetal small intestine. Endocrinology 102: 312–320, 1978.
 235. Lee, D. K. H., M., Stern, and S. Solomon. Cytoplasmic glucocorticoid receptors in the developing small intestine of the rabbit fetus. Endocrinology 99: 379–388, 1976.
 236. Lee, I. M. Coupar. Inhibition of intestinal secretion without reduction of cyclic AMP levels. Eur. J. Pharmacol. 68: 501–503, 1980.
 237. Lee, M. K., and I. M. Coupar. Inhibition by morphine of dibutyril cyclic AMP‐induced fluid secretion from the rat jejunum. J. Pharm. Pharmacol. 35: 253–255, 1983.
 238. Leeman, S. E., and R. Hammerschlag. Stimulation of salivary secretion by a factor extracted from hypothalamic tissue. Endocrinology 81: 803–810, 1967.
 239. Lehy, T., M. Dubrasquet, and S. Bonfils. Effect of somatostatin on normal and gastric‐stimulated cell proliferation in the gastric and intestinal mucosae of the rat. Digestion 19: 99–109, 1979.
 240. Lentze, M. J., P. C., Colony, and J. S. Trier. Glucocorticoid receptors in isolated intestinal epithelial cells in rats. Am. J. Physiol. 249 (Gastrointest. Liver Physiol. 12): G58–G65, 1985.
 241. Levin, R. J. The effects of hormones on the absorptive, metabolic and digestive functions of the small intestine. J. Endocrinol. 45: 315–348, 1969.
 242. Levitan, I. B., and W. B. Adams. Cyclic AMP modulation of a specific ion channel in an identified nerve cell: possible role of protein phosphorylation. Adv. Cyclic Nucleotide Res. 14: 647–653, 1981.
 243. Lichtenberger, L., L. R., Miller, D. N. Erwin, and L. R. Johnson. Effect of pentagastrin on adult rat duodenal cells in culture. Gastroenterology 65: 242–251, 1973.
 244. Lin, T. M. Pancreatic polypeptide: isolation, chemistry and biological function. In: Gastrointestinal Hormones, edited by G. B. Jerzy Glass, New York: Raven, 1980, p. 275–306.
 245. Linnoila, R. I., R. P., Diaugustine, R. J. Miller, K. J. Chang, and P. Cuatrecasas. An immunohistochemical and radioimmunological study of the distribution of (Met5)‐ and (Leu5)‐enkephalin in the gastrointestinal tract. Neuroscience 3: 1187–1191, 1978.
 246. Lipkin, M. Proliferation and differentiation of gastrointestinal cells. Physiol. Rev 53: 891–915, 1973.
 247. Lombes, M., M., Claire, M. Pinto, A. Michaud, and M. E. Rafestin‐Oblin. Aldosterone binding in the human colon carcinoma cell line HT‐29: correlation with cell differentiation. J. Steroid Biochem. 20: 329–334, 1984.
 248. López‐Ruiz, M. P., E., Arilla, L. Gonzalez‐Guuarro, and J. C. Prieto. Somatostatin binding sites in cytosolic fraction of rabbit intestinal mucosa: distribution throughout the intestinal tract. Comp. Biochem. Physiol. 81: 1041–1044, 1985.
 249. Lucid, S. W., and A. C. Cox. The effect of choleratoxin on the phosphorylation of protein in epithelial cells and their brush borders. Biochem. Biophys. Res. Commun. 49: 1183–1186, 1972.
 250. Lundberg, J. M., K., Tatemoto, L. Terenius, P. M. Hellström, V. Mutt, T. Hokfelt, and B. Hamberger. Localization of peptide YY (PYY) in gastrointestinal endocrine cells and effects on intestinal blood flow and motility. Proc. Natl. Acad. Sci. USA 79: 4471–4475, 1982.
 251. Mahmood, A., R. M., Pathak, and N. Agarwal. Effect of chronic alloxan diabetes and insulin administration on intestinal brush border enzymes. Experientia Basel 34: 741–742, 1977.
 252. Mailman, D. Effects of vasoactive intestinal polypeptide on intestinal absorption and blood flow. J. Physiol. Lond. 279: 121–132, 1978.
 253. Makhlouf, G. M. Distinct mechanism for stimulation of intestinal secretion by vasoactive intestinal peptide (VIP) and glucagon (Abstract). Gastroenterology 72: 368, 1977.
 254. Makhlouf, G. M. Role of VIP in the function of the gut. In: Advances of Peptide Hormone Research Series, edited by S. I. Said. New York: Raven, 1982, p. 425–446.
 255. Malmfors, G., S., Leander, E. Brodin, R. Håkanson, T. Holmin, and F. Sundler. Peptide‐containing neurons intrinsic to the gut wall. An experimental study in the pig. Cell Tissue Res. 214: 225–238, 1981.
 256. Mangeat, P., O., Ait‐Ahmed, G. Marchis‐Mouren, and J. Marvaldi. Studies on protein kinases in two human recto‐colic cell lines by Polyacrylamide gel electrophoresis. Effect of the vasoactive intestinal peptide. Biochim. Biophys. Acta 714: 320–330, 1982.
 257. Mangeat, P., J., Marvaldi, O. Ait‐Ahmed, and G. Marchis‐Mouren. Parallel activation of cyclic AMP phosphodiesterase and cyclic AMP‐dependent protein kinase in two human gut carcinoma cells (HT‐29 and HRT‐18) in culture, by vasoactive intestinal peptide (VIP) and other effectors activating the cyclic AMP system. Regul. Pept. 1: 397–414, 1981.
 258. Manning, D. C., S. H., Snyder, J. F. Kachur, R. J. Miller, and M. Field. Bradykinin receptor‐mediated chloride secretion in intestinal function. Nature Lond. 299: 256–259, 1982.
 259. Marie, J. C., D. Hui Bon, Hoa, G. Hejblum, and G. Rosselin. Cycle of VIP in the human transformed colonic epithelial cells (HT‐29) in culture (Abstract). Regul. Pept. Suppl. 3: S33, 1985.
 260. Marki, F. Effect of somatostatin on intestinal absorption of nutrients in the rat. Regul. Pept. 2: 371–381, 1981.
 261. Marvaldi, J., P., Mangeat, O. Ait‐Ahmed, C. Coeroli, and G. Marchis‐Mouren. Activation of cyclic AMP‐dependent protein kinases in human gut adenocarcinoma (HT‐29) cells in culture. Biochim. Biophys. Acta 588: 12–19, 1979.
 262. Marver, D. Assessment of mineralocorticoid activity in the rabbit colon. Am. J. Physiol. 246 (Renal Fluid Electrolyte Physiol. 15): F437–F446, 1984.
 263. Mashford, M. L., G., Nilsson, A. Rökaeus, and J. S. Rosell. The effect of food ingestion on circulating neurotensin‐like immunoreactivity (NTLI) in the human. Acta Physiol. Scand. 104: 244–246, 1978.
 264. Mcfadyen, R. J., J. M. Allen, and S. R. Bloom. NPY stimulates net absorption across rat intestinal mucosa in vivo. Neuropeptides 7: 219–228, 1986.
 265. Mckay, J. S., B. D., Linaker, and L. A. Turnberg. Influence of opiates on ion transport across rabbit ileal mucosa. Gastroenterology 80: 279–284, 1981.
 266. Menard, D., and C. Malo. Insulin‐evoked precocious appearance of intestinal sucrase activity in suckling mice. Dev. Biol. 69: 661–665, 1979.
 267. Menard, D., C., Malo, and R. Calvert. Insulin accelerates the development of intestinal brush border hydrolytic activities of suckling mice. Dev. Biol. 85: 150–155, 1981.
 268. Mitjavila, M. T., S., Mitjavila, and R. Derache. Mesures du métabolisme et de la lyse des cellules épitheliales isolées de l'intestin du rat incubées en présence de divers détergents. Toxicology 1: 237–248, 1973.
 269. Modlin, I. M., S. R., Bloom, and S. J. Mitchell. Experimental evidence for vasoactive intestinal peptide as the cause of the watery diarrhea syndrome. Gastroenterology 75: 1051–1054, 1978.
 270. Mountjoy, K. G., I. M., Holdaway, and G. J. Finlay. Insulin receptor regulation in cultured human tumor cells. Cancer Res. 43: 4537–4542, 1983.
 271. Muller, J. M., A., Elbattari, E. Ah‐Kye, J. Luis, F. Ducret, J. Pichon, and J. Marvaldi. Internalization of the vasoactive intestinal peptide (VIP) in a human adenocarcinoma cell line (HT‐29). Eur. J. Biochem. 152: 107–114, 1985.
 272. Muller, J. M., J., Luis, J. Fantini, B. Abadie, F. Giannellini, J. Marvaldi, and J. Pichon. Covalent cross‐linking of vasoactive intestinal peptide (VIP) to its receptor in intact colonic adenocarcinoma cells in culture (HT‐29). Eur. J. Biochem. 151: 411–417, 1985.
 273. Murer, H., U., Hopfer, and R. Kinne. Adenylate cyclase in the enterocyte: cellular localization and possible relation to transepithelial transport. In: Hormonal Receptors in Digestive Tract Physiology, edited by S. Bonfils, P. Fromageot, and G. Rosselin. Amsterdam: Elsevier/North‐Holland, 1977, p. 425–434.
 274. Mutt, V. Isolation and structure of vasoactive intestinal polypeptide from various species. In: Advances in Peptide Hormone Research Series, edited by S. I. Said. New York: Raven, 1982, vol. 1, p. 1–10.
 275. Mutt, V., J. E., Jorpes, and S. Magnusson. Structure of porcine secretin. The amino acid sequence. Eur. J. Biochem. 15: 513–519, 1970.
 276. Nakaki, T., T., Nakadate, S. Yamamoto, and R. Kato. Alpha2‐adrenergic inhibition of intestinal secretion induced by prostaglandins Ei, vasoactive intestinal peptide and dibutyryl cyclic AMP in rat jejunum. J. Pharmacol. Exp. Ther. 220: 637–641, 1982.
 277. Nakaki, T., T., Nakadate, S. Yamamoto, and R. Kato. Alpha2‐adrenergic receptor in intestinal epithelial cells. Identification by [3H]yohimbine and failure to inhibit cyclic AMP accumulation. Mol. Pharmacol. 23: 228–234, 1983.
 278. Neutra, M. R., L. J., O'Malley, and R. D. Specian. Regulation of intestinal goblet cell secretion. II. A survey of potential secretagogues. Am. J. Physiol. 242 (Gastrointest. Liver Physiol. 5): G380–G387, 1982.
 279. Oka, Y., F. K., Ghisman, H. L. Greene, and D. N. Orth. Effect of mouse epidermal growth factor/urogastrone on the functional maturation of rat intestine. Endocrinology 112: 940–944, 1983.
 280. Orci, L., O., Obaltens, C. Rufener, M. Brown, W. Vale, and R. Guillemin. Evidence for immunoreactive neurotensin in dog intestinal mucosa. Life Sci. 19: 559–562, 1976.
 281. Orwoll, E. S., and J. W. Kendall. Beta‐endorphin and adrenocorticotropin in extrapituitary sites: gastrointestinal tract. Endocrinology 107: 438–442, 1980.
 282. Pappas, T. N., H. T., Debas, Y. Goto, and I. L. Taylor. Peptide YY inhibits meal‐stimulated pancreatic and gastric secretion. Am. J. Physiol. 248 (Gastrointest. Liver Physiol. 11): G118–G123, 1985.
 283. Paris, C. G., V. F., Castelluci, E. R. Kandel, and J. H. Schwartz. Protein dephosphorylation, presynaptic facilitation, and behavioral sensitization in Aphysia. In: Protein Phosphorylation, edited by O. M. Rosen and E. G. Krebs. Cold Spring Harbor, NY: Cold Spring Harbor, 1981, p. 1361–1375. (Cold Spring Harbor Conf. Cell Proliferation 8.)
 284. Parker, D. S., J. P., Raufman, T. L. O'Donohue, M. Bledsoe, M. Yoshida, and J. J. Pisano. Aminoacid sequences of helospectins, new members of the glucagon superfamily, found in Gila monster venom. J. Biol. Chem. 259: 11751–11755, 1984.
 285. Pasternak, G. W., R., Goodman, and S. H. Snyder. An endogenous morphine‐like factor in mammalian brain. Life Sci. 16: 1765–1769, 1975.
 286. Perris, D. A. Isolation of the epithelial cells of the rat small intestine. Can. J. Biochem. 44: 687–693, 1966.
 287. Petith, M. M., and H. P. Schedl. Effects of diabetes on cecal and colonic calcium transport in the rat. Am. J. Physiol. 235 (Endocrinol. Metab. 4): E699–E702, 1978.
 288. Petrides, P. E., M., Hosang, E. Shooter, F. S. Esch, and P. Bohlen. Isolation and characterization of epidermal growth factor from human milk. FEBS Lett. 187: 89–95, 1985.
 289. Phillips, P. D., E., Kuhnle, and V. J. Cristofalo. 125I‐EGF binding ability is stable throughout the replicative life‐span of WI‐38 cells. J. Cell Physiol. 114: 311–316, 1983.
 290. Pichon, J., M., Hirn, J. M. Müller, P. Mangeat, and J. Marvaldi. Anti‐cell surface monoclonal antibodies which antagonize the action of VIP in a human adenocarcinoma cell line (HT‐29). EMBO J. 2: 1017–1022, 1983.
 291. Pillion, D. J., V., Ganapathy, and F. H. Leibach. Identification of insulin receptors on the mucosal surface of colon epithelial cells. J. Biol. Chem. 260: 5244–5247, 1985.
 292. Pinto, M., M. D., Appay, P. Simon‐Assmann, G. Chevalier, N. Dracopoli, J. Fogh, and A. Zweibaum. Enterocytic differentiation of cultured human colon cancer cells by replacement of glucose by galactose in the medium. Biol. Cell 44: 193–196, 1982.
 293. Pinto, M., S., Robine‐Leon, M. D. Appay, M. Kedinger, N. Triadou, E. Dussaulx, B. Lacroix, P. Simon‐Assmann, K. Haffen, J. Fogh, and A. Zweibaum. Enterocyte‐like differentiation and polarization of the human colon carcinoma cell line Caco‐2 in culture. Biol. Cell 47: 323–330, 1983.
 294. Polak, J. M., and S. R. Bloom. Neural and cellular origin of gastrointestinal hormone peptides in health and disease. In: Gastrointestinal Hormones, edited by G. B. Jerzy Glass. New York: Raven, 1980, p. 19–51.
 295. Polak, J. M., A. G. E., Pearse, L. Grimelius, S. R. Bloom, and A. Arimura. Growth hormone release‐inhibiting hormone in gastrointestinal and pancreatic D‐cells. Lancet 1: 1220–1222, 1975.
 296. Polak, J. M., S. N., Sullivan, S. R. Bloom, A. M. J. Buchan, P. Facer, M. R. Brown, and A. G. E. Pearse. Specific localization of neurotensin to N‐cell in human intestine by radioimmunoassay and immunohistochemistry. Nature Lond. 270: 183–184, 1977.
 297. Polak, J. M., S. N., Sullivan, S. R. Bloom, P. Facer, and A. G. E. Pearse. Enkephalin‐like immunoreactivity in the human gastrointestinal tract. Lancet 1: 972–974, 1977.
 298. Polonsky, K. S., S. E., Shoelson, and H. M. Docherty. Plasma somatostatin 28 increases in response to feeding in man. J. Clin. Invest. 71: 1514–1518, 1983.
 299. Pradayrol, L., J. A., Chayvialle, M. Carlquist, and V. Mutt. Isolation of a porcine intestinal peptide with C‐terminal somatostatin. Biochem. Biophys. Res. Commun. 85: 701–708, 1978.
 300. Pradayrol, L., M., Jörnvall, V. Mutt, and A. Ribet. N‐terminally extended somatostatin: the primary structure of so‐matostatin‐28. FEBS Lett. 209: 55–58, 1980.
 301. Prieto, J. C., J. M., Guerrero, and R. Goberna. No effect of starvation on vasoactive intestinal peptide (VIP) receptors in intestinal epithelial cells. Horm. Metab. Res. 12: 223–224, 1980.
 302. Prieto, J. C., M., Laburthe, D. Hui Bon Hoa, and G. Rosselin. Quantitative studies of vasoactive intestinal peptide (VIP) binding sites and VIP‐induced adenosine 3':5'‐monophosphate production in epithelial cells from duodenum, jejunum, ileum, coecum, colon and rectum in the rat. Acta Endocrinol. 96: 100–106, 1981.
 303. Prieto, J. C., M., Laburthe, and G. Rosselin. Interaction of vasoactive intestinal peptide with isolated intestinal epithelial cells from rat. I. Characterization, quantitative aspects and structural requirements of binding sites. Eur. J. Biochem. 96: 229–237, 1979.
 304. Prinz, R. A., L., Kirsteins, E. Connick, E. Paloyan, and A. M. K. Lawrence. Insulin and glucagon in human pancreatic exocrine fluid. Horm. Metab. Res. 12: 38–42, 1980.
 305. Quaroni, A., and R. J. May. Establishment and characterization of intestinal epithelial cell cultures. Methods Cell Biol. 21: 403–427, 1980.
 306. Quaroni, A., J., Wands, R. L. Trelstad, and K. J. Isselbacher. Epithelioid cell cultures from rat small intestine. Characterization by morphologic and immunologic criteria. J. Cell Biol. 80: 248–265, 1979.
 307. Rachmilewitz, O., F., Karmeli, M. Chorev, and Z. Selinger. Effect of opiates on human colonic adenylate cyclase activity. Eur. J. Pharmacol. 93: 169–173, 1983.
 308. Racusen, L. C., and H. J. Binder. Alteration of large intestinal electrolyte transport by vasoactive intestinal polypeptide in the rat. Gastroenterology 73: 790–796, 1977.
 309. Rafestin‐Oblin, M. E., M., Lombes, J. B. Michel, A. Michaud, and M. Claire. Mineralocorticoid receptors in the epithelial cells of human colon and ileum. J. Steroid Biochem. 20: 311–315, 1984.
 310. Ramond, M. J., M., Martinot‐Peignoux, and S. Erlinger. Dome formation in the human colon carcinoma cell line Caco‐2 in culture. Influence of ouabain and permeable supports. Biol. Cell 54: 89–92, 1985.
 311. Raul, F., M., Kedinger, P. Simon, J. Grenier, and K. Happen. Behaviour of isolated rat intestinal cells maintained in suspension or monolayer cultures. Biol. Cell 33: 163–168, 1978.
 312. Raul, F., P., Simon, M. Kedinger, and K. Haffen. Intestinal enzymes activities in isolated villus and crypt cells during postnatal development of the rat. Cell Tissue Res. 176: 167–178, 1977.
 313. Reyl, F., and M. J. M. Lewin. Somatostatin is a potent activator of phosphoprotein phosphatases in the digestive tract. Biochim. Biophys. Acta 675: 297–300, 1981.
 314. Reyl, F. J., and M. J. M. Lewin. Intracellular receptor for somatostatin in gastric mucosa cells: decomposition and reconstitution of somatostatin‐stimulated phosphoprotein phosphatases. Proc. Natl. Acad. Sci. USA 79: 978–982, 1982.
 315. Reyl‐Desmars, F., and M. J. M. Lewin. Evidence for an intracellular somatostatin receptor in pancreas: a comparative study with reference to gastric mucosa. Biochem. Biophys. Res. Commun. 109: 1324–1331, 1982.
 316. Robine, S., C., Huet, R. Moll, C. Sahuquillo‐Merino, E. Coudrier, A. Zweibaum, and D. Louvard. Can villin be used to identify malignant and undifferentiated normal digestive epithelial cells? Proc. Natl. Acad. Sci. USA 82: 8488–8492, 1985.
 317. Rodbell, M., L., Birnbaumer, S. Pohl, and M. J. Krans. The glucagon‐sensitive adenylate cyclase system in plasma membranes of rat liver. V. An obligatory role of guanyl nucleotides in glucagon action. J. Biol. Chem. 246: 1877–1882, 1971.
 318. Roomi, N., M., Laburthe, N. Fleming, R. Crowther, and J. Forstner. Cholera‐induced mucin secretion from rat intestine: lack of effect of cAMP, cycloheximide, VIP, and colchicine. Am. J. Physiol. 247 (Gastrointest. Liver Physiol. 10): G140–G148, 1984.
 319. Rosell, S., and A. Rökaeus. The effect of ingestion of amino acids, glucose and fat on circulating neurotensin‐like immunoreactivity (NTLI) in man. Acta Physiol. Scand. 107: 263–267, 1979.
 320. Rosselin, G., W., Rosztejn, M. Laburthe, and P. M. Dubois. Is VIP a neuroregulator or a hormone? In: Hormonally Active Brain Peptides, edited by K. W. McKerns and V. Pantic. New York: Plenum, 1982, p. 367–395.
 321. Rouiller, D., V., Schusdziarra, J. M. Conlon, V. Harris, and R. H. Unger. Release of somatostatin‐like immunoreactivity from the lower gut. Gastroenterology 77: 700–703, 1979.
 322. Rousset, M., M., Laburthe, G. Chevalier, C. Boissard, G. Rosselin, and A. Zweibaum. Vasoactive intestinal peptide (VIP) control of glycogenolysis in the human colon carcinoma cell line HT‐29 in culture. FEBS Leu. 126: 38–40, 1981.
 323. Rousset, M., M., Laburthe, M. Pinto, G. Chevalier, C. Rouyer‐Fessard, E. Dussaulx, G. Trugnan, N. Boige, J. L. Brun, and A. Zweibaum. Enterocytic differentiation and glucose utilization in the human colon tumor cell line Caco‐2: modulation by forskolin. J. Cell. Physiol. 123: 377–385, 1985.
 324. Sahyoun, N., R. A., Hoch, and M. D. Hollenberg. Insulin and epidermal growth factor‐urogastrone: affinity cross‐linking to specific binding sites in rat liver membranes. Proc. Natl. Acad. Sci. USA 75: 1675–1679, 1978.
 325. Said, S. I. Vasoactive intestinal peptide (VIP): isolation, distribution, biological actions, structure‐function relationships, and possible functions. In: Gastrointestinal Hormones, edited by G. B. Jerzy Glass. New York: Raven, 1980, p. 245–273.
 326. Said, S. I., and G. R. Faloona. Elevated plasma and tissue levels of vasoactive intestinal peptide in the watery diarrhea syndrome. N. Engl. J. Med. 293: 155–160, 1975.
 327. Said, S. I., and V. Mutt. Polypeptide with broad biological activity: isolation from rat small intestine. Science Wash. DC 169: 1217–1218, 1970.
 328. Said, S. I., and V. Mutt. Potent peripheral and splanchnic vasodilatator peptide from normal gut. Nature Lond. 225: 863–864, 1970.
 329. Sakurai, H., R., Dobbs, and R. H. Unger. Somatostatin‐induced changes in insulin and glucagon secretion in normal and diabetic dogs. J. Clin. Invest. 54: 1395–1402, 1974.
 330. Saria, A., and E. Beubler. Neuropeptide Y (NPY) and peptide YY (PYY) inhibit prostaglandin E2‐induced intestinal fluid and electrolyte secretion in the rat jejunum in vivo. Eur. J. Pharmacol. 119: 47–52, 1985.
 331. Sarrieau, A., N., Boige, and M. Laburthe. Role of phospholipids in the binding activity of vasoactive intestinal peptide receptors. Experientia Basel 41: 631–633, 1985.
 332. Sarrieau, A., M., Laburthe, and G. Rosselin. Intestinal VIP receptors: differential effect of trypsin on the high and low affinity bindings sites. Mol. Cell. Endocrinol. 31: 301–313, 1983.
 333. Savage, C. R., T., Inagami, and S. Cohen. The primary structure of epidermal growth factor. J. Biol. Chem. 247: 7612–7621, 1972.
 334. Scalera, V., J., Biber, and H. Murer. The effect of cyclic nucleotides and cholera toxin on in vivo and in vitro dephosphorylation of small intestinal brush border membranes. Biochim. Biophys. Acta 730: 321–326, 1983.
 335. Scemama, J. L., C., Ruellan, P. Clerc, F. Clemente, and A. Ribet. Dopamine receptors in a human colonic cancer cell line (HT‐29). Some receptors‐related biological effects of dopamine. Int. J. Cancer 34: 675–680, 1984.
 336. Schaffalitzky De Muckadell, O. B., J. Fahrenkrug, J. J. Holst, and K. B. Lauritsen. Release of vasoactive intestinal polypeptide (VIP) by intraduodenal stimuli. Scand. J. Gastroenterol. 12: 793–799, 1977.
 337. Schaffalitzky De Muckadell, O. B., J. Fahrenkrug, J. J. Holst, and K. B. Lauritsen. Release of vasoactive intestinal polypeptide (VIP) by electrical stimulation of the vagal nerves. Gastroenterology 72: 373–375, 1977.
 338. Schally, A. V., A., Dupont, A. Arimura, T. W. Redding, N. Nishi, G. L. Linthicum, and D. H. Schlesinger. Isolation and structure of somatostatin from porcine hypothalami. Biochemistry 15: 509–514, 1976.
 339. Schally, A. V., W., Huang, R. Chang, A. Arimura, T. W. Redding, R. P. Millar, M. W. Hunkapiller, and L. E. Hood. Isolation and structure of pro‐somatostatin: a putative somatostatin precursor from pig hypothalamus. Proc. Natl. Acad. Sci. USA 77: 4489–4493, 1980.
 340. Scheving, L. A., L. E., Scheving, T. H. Tsai, and J. E. Pauly. Circadian stage‐dependent effects of insulin and glucagon on incorporation of [3H]thymidine into deoxyribonucleic acid in the esophagus, stomach, duodenum, jejunum, ileum, caecum, colon, rectum and spleen of the adult female mouse. Endocrinology 111: 308–315, 1982.
 341. Scheving, L. A., Y. C., Yeh, T. H. Tsai, and L. E. Scheving. Circadian phase‐dependent stimulatory effects of epidermal growth factor on deoxyribonucleic acid synthesis in the tongue, esophagus and stomach of the adult male mouse. Endocrinology 105: 1475–1480, 1979.
 342. Scheving, L. A., Y. C., Yeh, T. H. Tsai, and L. E. Scheving. Circadian phase‐dependent stimulatory effects of epidermal growth factor on deoxyribonucleic acid synthesis in the duodenum, jejunum, ileum, caecum, colon, and rectum of the adult male mouse. Endocrinology 106: 1498–1503, 1980.
 343. Schlegel, W., S., Raptis, H. C. Dollinger, and, F. Pfeiffer. Inhibition of secretin, pancreozymin and gastrin release and their biological activities by somatostatin. In: Hormonal Receptors in Digestive Tract Physiology, edited by S. Bonfils, P. Fromageot, and G. Rosselin. Amsterdam: Elsevier/North‐Holland, 1977, p. 361–377.
 344. Schlessinger, J., and B. Geiger. Epidermal growth factor induces redistribution of actin and actinin in human epidermal carcinoma cells. Exp. Cell Res. 134: 273–279, 1981.
 345. Schneider, L. E., L. M., Nowosielski, and H. P. Schedl. Insulin‐treatment of diabetic rats: effects on duodenal calcium absorption. Endocrinology 100: 67–73, 1977.
 346. Schramm, M., J., Orly, S. Eimerl, and M. Korner. Coupling of hormone receptors to adenylate cyclase of different cells by cell fusion. Nature Lond. 268: 310–313, 1977.
 347. Schultz, S. G. Chloride transport by gastrointestinal epithelia: an overview. In: Mechanisms of Intestinal Secretion, edited by H. J. Binder. New York: Liss, p. 93–99. (Kroc Found. Ser. 12.)
 348. Schultzberg, M., C. F., Dreyfus, M. D. Gershon, T. Hökfelt, R. Elde, G. Nilsson, S. Said, and M. Goldstein. VIP‐enkephalin‐, substance P‐, and somatostatin‐like immunoreactivity in neurone intrinsic to the intestine: immunohistochemical evidence from organotypic tissue cultures. Brain Res. 155: 239–248, 1978.
 349. Schultzberg, M., T., Hökfelt, G. Nilsson, L. Terenius, J. F. Rehfeld, M. Brown, R. Elde, M. Goldstein, and S. Said. Distribution of peptide‐ and catecholamine‐containing neurons in the gastrointestinal tract of the rat and guinea pig: immunohistochemical studies with antisera to substance P, vasoactive intestinal polypeptide, enkephalins, somatostatin, gastrin/cholecystokinin, neurotensin and dopamine beta‐hydroxylase. Neuroscience 5: 689–744, 1980.
 350. Schusdziarra, V. Somatostatin: a regulatory modulator connecting nutrient entry and metabolism. Horm. Metab. Res. 12: 563–577, 1980.
 351. Schwartz, C. J., D. V., Kimberg, H. E. Sheerin, M. Field, and S. I. Said. Vasoactive intestinal peptide stimulation of adenylate cyclase and active electrolyte secretion in intestinal mucosa. J. Clin. Invest. 54: 536–544, 1974.
 352. Sharp, G. W. G., M. E., Cohen, R. Gudewich, L. Taylor, D. Button, D. Albrewczynski, and M. Donowitz. Protein phosphorylation and the control of intestinal electrolyte transport. In: Mechanisms of Intestinal Electrolyte Transport and Regulation by Calcium, edited by A. R. Liss. New York: Liss, 1984, p. 241–261.
 353. Shlatz, L. J., D. V., Kimberg, and K. A. Cattieu. Cyclic nucleotide‐dependent phosphorylation of rat intestinal microvillus and basalateral membrane proteins by an endogenous protein kinase. Gastroenterology 75: 838–846, 1978.
 354. Shlatz, K. J., D. V., Kimberg, and K. A. Cattieu. Phosphorylation of specific rat intestinal microvillus and basal‐lateral membrane by cyclic nucleotides. Gastroenterology 76: 293–298, 1979.
 355. Simantov, R., and S. H. Snyder. Isolation and structure identification of a morphine‐like peptide “enkephalin” in bovine brain. Life Sci. 18: 781–788, 1976.
 356. Simon, B., and H. Kather. Activation of human adenylate cyclase in the upper gastrointestinal tract by vasoactive intestinal polypeptide. Gastroenterology 74: 722–725, 1978.
 357. Simon, B., and H. Kather. Human colonic adenylate cyclase. Stimulation of enzyme activity by vasoactive intestinal peptide and various prostaglandins via distinct receptor sites. Digestion 20: 62–67, 1980.
 358. Simon‐Assmann, P. M., M., Kedinger, J. F. Grenier, and K. Haffen. Control of brush border enzymes by dexamethasone in the fetal rat intestine cultured in vitro. J. Pediatr. Gastroenterol. Nutr. 1: 257–265, 1982.
 359. Sjöstrand, F. S. A simple and rapid method to prepare dispersions of columnar epithelial cells from the rat intestine. J. Ultrastruct. Res. 22: 424–442, 1968.
 360. Skov Olsen, P., and E. Nexo. Quantitation of epidermal growth factor in the rat. Identification and partial characterization of duodenal EGF. Scand. J. Gastroenterol. 18: 771–776, 1983.
 361. Smith, T. W., J., Hughes, H. W. Kosterlitz, and R. P. Sosa. Enkephalins: isolation, distribution and function. In: Opiates and Endogenous Opioid Peptides, edited by H. W. Kosterlitz. Amsterdam: Elsevier/North‐Holland, 1976, p. 57–62.
 362. Sodoyez‐Goffaux, F., J. C., Sodoyez, and C. J. Devos. Insulin receptors in the gastrointestinal tract of the rat fetus: quantitative autoradiographic studies. Diabetologia 28: 45–50, 1985.
 363. Solomon, S., and D. K. H. Lee. Binding of glucocorticoids in fetal tissues. J. Steroid Biochem. 8: 453–461, 1977.
 364. Solomon, T. E. Pancreatic polypeptide, peptide YY, and neuropeptide Y family of regulatory peptides. Gastroenterology 88: 838–844, 1985.
 365. Sonne, O. Receptor‐mediated degradation and internalization of insulin in the adenocarcinoma cell line HT‐29 from human colon. Mol. Cell. Endocrinol. 39: 39–48, 1985.
 366. Spiess, J., J., Rivier, V. Thorner, and W. Vale. Sequence analysis of a growth hormone‐releasing factor from a pancreatic islet tumor. Biochemistry 21: 6037–6040, 1982.
 367. Stange, F. E., A., Schneider, V. Schusdziarra, and H. Dit‐Schuneit. Inhibitory effects of somatostatin on growth and differentiation in cultured intestinal mucosa. Horm. Metab. Res. 16: 74–78, 1984.
 368. Stern, B. K., and W. E. Jensen. Active transport of glucose by suspension of isolated rat intestinal epithelial cells. Nature Lond. 209: 789–790, 1966.
 369. Suchaneck, G., and G. Kreil. Translation of melittin messenger RNA in vitro yields a product terminating with glutaminylglycine rather than glutaminamide. Proc. Natl. Acad. Sci. USA 74: 975–978, 1977.
 370. Sundler, F., J., Alumets, K. J. Chang, and R. Håkanson. Cellular localization and distribution of Leu‐enkephalin immunoreactivity in the gut (Abstract). Scand. J. Gastroenterol. Suppl. 49: 13, 1978.
 371. Sundler, F., E., Moghimzadeh, R. Håkanson, M. Ekelund, and P. C. Emson. Nerve fibers in the gut and pancreas of the rat displaying neuropeptide Y immunoreactivity. Intrinsic and extrinsic origin. Cell Tissue Res. 230: 487–493, 1983.
 372. Tachibana, S., K. Arakai, S. Ohya, and S. Yoshida. Isolation and structure of dynorphin, an opioid peptide, from porcine duodenum. Nature Lond. 295: 339–340, 1982.
 373. Tange, A. Distribution of peptide‐containing endocrine cells and neurons in the gastrointestinal tract of the dog: immunohistochemical studies using antisera to somatostatin, substance P, vasoactive intestinal polypeptide, Met‐enkephalin, and neurotensin. Biomed. Res. 4: 9–24, 1983.
 374. Taparel, D., J. P., Esteve, C. Susini, N. Vaysse, D. Balas, G. Berthon, E. Wunsch, and A. Ribet. Binding of somatostatin to guinea pig pancreatic membranes: regulation by ions. Biochem. Biophys. Res. Commun. 3: 827–833, 1983.
 375. Tapper, E. J. Local modulation of intestinal ion transport by enteric neurons. Am. J. Physiol. 244 (Gastrointest. Liver Physiol. 7): G457–G468, 1983.
 376. Tapper, E. J., A. S., Bloom, and D. L. Lewand. Endogenous norepinephrine release induced by tyramine modulates intestinal ion transport. Am. J. Physiol. 241 (Gastrointest. Liver Physiol. 4): G264–G269, 1981.
 377. Tapper, E. J., D. W., Powell, and S. M. Morris. Cholinergic‐adrenergic interactions on intestinal ion transport. Am. J. Physiol. 235 (Endocrinol. Metab. Gastrointest. Physiol. 4): E402–E409, 1978.
 378. Tatemoto, K. Isolation and characterization of peptide YY (PYY), a candidate gut hormone that inhibits pancreatic exocrine secretion. Proc. Natl. Acad. Sci. USA 79: 2514–2518, 1982.
 379. Tatemoto, K. Neuropeptide Y: complete amino acid sequence of the brain peptide. Proc. Natl. Acad. Sci. USA 79: 5485–5489, 1982.
 380. Tatemoto, K., M., Carlquist, and V. Mutt. Neuropeptide Y. A novel brain peptide with structural similarities to peptide YY and pancreatic polypeptide. Nature Lond. 296: 659–660, 1982.
 381. Tatemoto, K., and V. Mutt. Chemical determination of polypeptide hormones. Proc. Natl. Acad. Sci. USA 75: 4115–4119, 1978.
 382. Tatemoto, K., and V. Mutt. Isolation of two novel candidate hormones using a chemical method for finding natural occurring polypeptides. Nature Lond. 285: 417–418, 1980.
 383. Tatemoto, K., and V. Mutt. Isolation and characterization of the intestinal porcine (PHI‐27) a new member of the glucagon‐secretin family. Proc. Natl. Acad. Sci. USA 78: 6603–6607, 1981.
 384. Taylor, I. L. Distribution and release of peptide YY in dog measured by specific radioimmunoassay. Gastroenterology 88: 731–737, 1985.
 385. Taylor, L., V. J. Guerina, M. Donowitz, M. Cohen, and G. W. G. Sharp. Calcium and calmodulin‐dependent protein phosphorylation in rabbit ileum. FEBS Lett. 131: 322–324, 1981.
 386. Thornburg, W., L., Matrisian, B. Magun, and O. Koldovský. Gastrointestinal absorption of epidermal growth factor in suckling rats. Am. J. Physiol. 246 (Gastrointest. Liver Physiol. 9): G80–G85, 1984.
 387. Tien, X. Y., R., Wahawisan, L. J. Wallace, and T. S. Gaginella. Intestinal epithelial cells and musculature contain different muscarinic binding sites. Life Sci. 36: 1949–1955, 1985.
 388. Trier, J. S. Morphology of the epithelium of the small intestine. In: Handbook of Physiology. Alimentary Canal. Intestinal Absorption, edited by C. F. Code. Washington, DC: Am. Physiol. Soc., 1968, sect. 6, vol. 3, chapt. 63, p. 1125–1175.
 389. Tsukada, T., S. J., Horovitch, M. R. Montminy, G. Mandel, and R. H. Goodman. Structure of the human vasoactive intestinal polypeptide gene. DNA NY 4: 293–300, 1985.
 390. Uhl, G. R., J. P., Bennett, and S. H. Snyder. Neurotensin, a central nervous system peptide: apparent receptor binding in brain membranes. Brain Res. 130: 299–313, 1977.
 391. Unger, R. H., R. E., Dobbs, and L. Orci. Insulin, glucagon and somatostatin secretion in the regulation of metabolism. Annu. Rev. Physiol. 40: 307–343, 1978.
 392. Van Neuten, J. N., J. M. Van Ree, and P. M. Vanhoutte. Inhibition by met‐enkephalin of peristaltic activity in the guinea pig ileum and its reversal by naloxone. Eur. J. Pharmacol. 41: 341–342, 1977.
 393. Verner, J. V., and A. B. Morrison. Islet cell tumor and a syndrome of refractory diarrhea and hypokalemia. Am. J. Med. 25: 373–380, 1958.
 394. Vinik, A. I., T. S., Gaginella, T. M. O'Dorisio, B. Shapiro, and L. Wagner. The distribution and characterization of somatostatin‐like immunoreactivity in epithelial cells, sub‐mucosa and muscle of the rat stomach and intestine. Endocrinology 109: 1921–1926, 1981.
 395. Vlodavsky, I., K. D., Brown, and D. Gospodarowicz. A comparison of the binding of epidermal growth factor to cultured granulosa and luteal cells. J. Biol. Chem. 253: 3744–3750, 1978.
 396. Waldman, D. B., J. D., Gardner, A. M. Zfass, and G. M. Makhloup. Effects of vasoactive intestinal peptide, secretin and related peptides on rat colonic transport and adenylate cyclase activity. Gastroenterology 73: 518–523, 1977.
 397. Walling, M. W., A. K., Mircheff, C. H. Van Os, and E. M. Wright. Subcellular distribution of nucleotide cyclases in rat intestinal epithelium. Am. J. Physiol. 235 (Endocrinol. Metab. Gastrointest. Physiol. 4): E539–E545, 1978.
 398. Watford, M., P., Lund, and H. A. Krebs. Isolation and metabolic characteristics of rat and chicken enterocytes. Biochem. J. 178: 589–596, 1979.
 399. Watts, R. W., and J. F. Wheldrake. Aldosterone induced changes in RNA synthesis in rat intestine. J. Steroid Biochem. 7: 263–266, 1976.
 400. Webster, H. L., and D. D. Harrison. Enzymic activities during the transformation of crypt to columnar intestinal cells. Exp. Cell Res. 56: 245–253, 1969.
 401. Weiser, M. M. Intestinal epithelial cell surface membrane glycoprotein synthesis. I. An indicator of cellular differentiation. J. Biol. Chem. 248: 2536–2541, 1973.
 402. Wiklund, B., L., Liljeqvist, and A. Rokaeus. Neurotensin increases net fluid secretion and transit rate in the small intestine of man. Regul. Pept. 8: 33–39, 1984.
 403. Wilen, T., S., Gustavsson, and B. Jung. Effects of neurotensin on small bowel propulsion in intact and vagotomized rats. Regul. Pept. 4: 191–201, 1982.
 404. Wolf, D. J., and C. O. Brostrom. Properties and functions of the calcium‐dependent regulator protein. Adv. Cyclic Nucleotide Res. 11: 27–88, 1979.
 405. Wolfe, M. M., R. I., Misbin, D. F. Gardner, and J. E. Mcguigan. Enteric release of vasoactive intestinal peptide after a peptone meal in the dog. Regul. Pept. 5: 103–109, 1983.
 406. Wong, M., and I. M. Holdaway. Insulin binding by normal and neoplastic colon tissue. Int. J. Cancer 35: 335–341, 1985.
 407. Wood, C. L., and M. S. O'Dorisio. Covalent cross‐linking of vasoactive intestinal polypeptide to its receptors on intact human lymphoblasts. J. Biol. Chem. 260: 1243–1247, 1985.
 408. Wu, Z. C., T. M., O'Dorisio, S. Cataland, H. S. Mekhjian, and T. S. Gaginella. Effects of pancreatic polypeptide and vasoactive intestinal polypeptide on rat ileal and colonic water and electrolyte transport in vivo. Dig. Dis. Sci. 24: 625–630, 1979.
 409. Yanaihara, N., K., Nokihara, C. Yanaihara, T. Iwanaga, and T. Fujita. Immunocytochemical demonstration of PHI and its co‐existence with VIP in intestinal nerves of the rat and pig. Arch. Histol. Jpn. 46: 575–581, 1983.
 410. Yousef, I. M., and A. Kuksis. Release of chylomicrons by isolated cells of rat intestinal mucosa. Lipids 7: 380–386, 1972.
 411. Zimmerman, T. W., and H. J. Binder. Muscarinic receptors on rat isolated colonic epithelial cells. A correlation between inhibition of [3H]quinuclidinyl benzylate binding and alteration in ion transport. Gastroenterology 83: 1244–1251, 1982.
 412. Zimmerman, T. W., J. W., Dobbins, and H. J. Binder. Mechanism of cholinergic regulation of electrolyte transport in rat colon in vitro. Am. J. Physiol. 242 (Gastrointest. Liver Physiol. 5): G116–G123, 1982.
 413. Ziomek, C. A., S., Schulman, and M. Edidin. Redistribution of membrane proteins in isolated mouse intestinal epithelial cells. J. Cell Biol. 86: 849–857, 1980.
 414. Zweibaum, A. Enterocytic differentiation of cultured human colon cancer cell lines: negative modulation by D‐glucose. In: Ion Gradient‐Coupled Transport, edited by F. Alvarado and P. Van Os. Amsterdam: Elsevier, 1986, p 345–353. (INSERM Symp. No. 26.)
 415. Zweibaum, A., M., Pinto, G. Chevalier, E. Dussaulx, N. Triadou, B. Lacroix, K. Haffen, J. L. Brun, and M. Rousset. Enterocytic differentiation of a subpopulation of the human colon tumor cell line HT‐29 selected for growth in sugar‐free medium and its inhibition by glucose. J. Cell. Physiol. 122: 21–29, 1985.

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Article Title: Peptide Receptors in Intestinal Epithelium
 

How to Cite

Marc Laburthe, Brigitte Amiranoff. Peptide Receptors in Intestinal Epithelium. Compr Physiol 2011, Supplement 17: Handbook of Physiology, The Gastrointestinal System, Neural and Endocrine Biology: 215-243. First published in print 1989. doi: 10.1002/cphy.cp060211