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Receptors for Gut Peptides on Smooth Muscle Cells of the Gut

Gabriel M. Makhlouf, John R. Grider

10.1002/cphy.cp060213

Source: Supplement 17: Handbook of Physiology, The Gastrointestinal System, Neural and Endocrine Biology

Originally published: 1989

Published online: January 2011

Full Article on Wiley Online Library



Abstract

The sections in this article are:

1 Receptors for Cholecystokinin
2 Receptors for Tachykinins
3 Receptors for Opioid Peptides
4 Receptors for Bombesins
5 Receptors for Vasoactive Intestinal Peptide
6 Receptors for Neuropeptide Y
7 Receptors for Calcitonin Gene‐Related Peptide
8 Receptors for Galanin
9 Receptors for Somatostatin
10 Receptors for Motilin
Figure 1. Figure 1.

Concentration‐response curves for contractile effects of cholecystokinin octapeptide [CCK‐OP (CCK‐8)] (closed circles), gastrin‐17 (closed squares), and acetylcholine (open circles) in isolated muscle cells from guinea pig stomach. CCK‐8 and gastrin‐17 are equipotent in gastric muscle cells. Responses in muscle cells from human stomach are identical.

From Bitar and Makhlouf 10
Figure 2. Figure 2.

Inhibition of contractile response of muscle cells isolated from gallbladder of dog by a preferential antagonist of substance P, [D‐Pro2,D‐Trp7,9]SP ([PTT]SP), and a preferential antagonist of bombesin, [D‐Arg1,D‐Pro2,D‐Trp7,9,Leu11]SP ([APTTL]SP). Differential effects of 2 antagonists are consistent with presence of separate receptors for SP and bombesin.

From Severi et al. 69
Figure 3. Figure 3.

Inhibition of specific binding of 125I‐labeled substance P by mammalian tachykinins and the substance P (SP) derivative, [D‐Pro2,D‐Trp7,9]SP, in isolated cells from longitudinal muscle layer of guinea pig small intestine. Same order of potency prevails when 125I‐labeled substance K is used as radioligand.

From Souquet et al. 72
Figure 4. Figure 4.

Concentration‐response curves for contractile effects of opioid peptides, dynorphin (DYN), Met‐enkephalin (MET‐ENK), Met‐enkephalin‐Arg6‐Phe7 (MEAP), β‐neoendorphin (β‐NEO‐E), and Leu‐enkephalin (LEU‐ENK) on smooth muscle cells isolated separately from circular and longitudinal muscle layers of guinea pig small intestine. Identical pattern of selective response in circular muscle cells is found in human intestine.

From Bitar and Makhlouf 13
Figure 5. Figure 5.

Concentration‐response curves for contractile effects of amphibian bombesin‐14 and 3 mammalian bombesins, neuromedin B, gastrin‐releasing peptide (GRP1–27), and the COOH‐terminal decapeptide of GRP (GRP18–27) on muscle cells isolated from circular muscle layer of human jejunum.

From Micheletti et al. 60
Figure 6. Figure 6.

Concentration‐response curves for relaxant effect of vasoactive intestinal peptide (VIP), alone and in combination with a threshold (open squares) and suprathreshold (open circles) concentration of isobutyl methylxanthine (IBMX).

From Bitar and Makhlouf 11


Figure 1.

Concentration‐response curves for contractile effects of cholecystokinin octapeptide [CCK‐OP (CCK‐8)] (closed circles), gastrin‐17 (closed squares), and acetylcholine (open circles) in isolated muscle cells from guinea pig stomach. CCK‐8 and gastrin‐17 are equipotent in gastric muscle cells. Responses in muscle cells from human stomach are identical.

From Bitar and Makhlouf 10


Figure 2.

Inhibition of contractile response of muscle cells isolated from gallbladder of dog by a preferential antagonist of substance P, [D‐Pro2,D‐Trp7,9]SP ([PTT]SP), and a preferential antagonist of bombesin, [D‐Arg1,D‐Pro2,D‐Trp7,9,Leu11]SP ([APTTL]SP). Differential effects of 2 antagonists are consistent with presence of separate receptors for SP and bombesin.

From Severi et al. 69


Figure 3.

Inhibition of specific binding of 125I‐labeled substance P by mammalian tachykinins and the substance P (SP) derivative, [D‐Pro2,D‐Trp7,9]SP, in isolated cells from longitudinal muscle layer of guinea pig small intestine. Same order of potency prevails when 125I‐labeled substance K is used as radioligand.

From Souquet et al. 72


Figure 4.

Concentration‐response curves for contractile effects of opioid peptides, dynorphin (DYN), Met‐enkephalin (MET‐ENK), Met‐enkephalin‐Arg6‐Phe7 (MEAP), β‐neoendorphin (β‐NEO‐E), and Leu‐enkephalin (LEU‐ENK) on smooth muscle cells isolated separately from circular and longitudinal muscle layers of guinea pig small intestine. Identical pattern of selective response in circular muscle cells is found in human intestine.

From Bitar and Makhlouf 13


Figure 5.

Concentration‐response curves for contractile effects of amphibian bombesin‐14 and 3 mammalian bombesins, neuromedin B, gastrin‐releasing peptide (GRP1–27), and the COOH‐terminal decapeptide of GRP (GRP18–27) on muscle cells isolated from circular muscle layer of human jejunum.

From Micheletti et al. 60


Figure 6.

Concentration‐response curves for relaxant effect of vasoactive intestinal peptide (VIP), alone and in combination with a threshold (open squares) and suprathreshold (open circles) concentration of isobutyl methylxanthine (IBMX).

From Bitar and Makhlouf 11
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Article Title: Receptors for Gut Peptides on Smooth Muscle Cells of the Gut
 

How to Cite

Gabriel M. Makhlouf, John R. Grider. Receptors for Gut Peptides on Smooth Muscle Cells of the Gut. Compr Physiol 2011, Supplement 17: Handbook of Physiology, The Gastrointestinal System, Neural and Endocrine Biology: 281-289. First published in print 1989. doi: 10.1002/cphy.cp060213