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Long‐Term Regulation of Pancreatic Function Studied in Vitro

Craig D. Logsdon

10.1002/cphy.cp060326

Source: Supplement 18: Handbook of Physiology, The Gastrointestinal System, Salivary, Gastric, Pancreatic, and Hepatobiliary Secretion

Originally published: 1989

Published online: January 2011

Full Article on Wiley Online Library



Abstract

The sections in this article are:

1 Long‐Term in Vitro Models of The Exocrine Pancreas
1.1 Organ Explant Cultures
1.2 Isolated Cell Cultures
1.3 Pancreatic Tumors and Cell Lines
2 Physiological Regulation of Functions Studied In Long‐Term in Vitro Systems
2.1 Regulation of Differentiation: Effects of Factors on the Synthesis of Secretory Enzymes
2.2 Regulation of Growth
2.3 Regulation of Secretion
2.4 Regulation of Hormone Receptors
3 Conclusions
Figure 1. Figure 1.

Light micrograph of cross section of 14‐day‐old pancreatic cultures on collagen gels. Cells form a monolayer of cuboidal cells with obvious polarity. Bar, 20 μm. X 1,100.

From Logsdon and Williams 55
Figure 2. Figure 2.

Electron micrograph of a typical cell from a 14‐day‐old pancreatic culture in cross section. Bar, 5 μm. X 3,600.

From Logsdon and Williams 55
Figure 3. Figure 3.

Time course of the dexamethasone‐induced increase in amylase concentration in AR42J cells. Dexamethasone 10 nM) was incubated with AR42J cells for up to 72 h. Cells were then analyzed for contents of amylase and protein. Values are units of amylase per milligrams of total protein and are means +L SD.

From Logsdon et al. 50, by copyright permission of the Rockefeller University Press
Figure 4. Figure 4.

Concentration dependence of amylase release stimulated by cholecystokinin octapeptide (CCK‐8) for AR42J cells raised for 48 h in the absence (open circles) or presence (filled circles) of 10 nM dexamethasone. Amylase release of 40 min is plotted as a function of concentration of CCK‐8 in the medium. A: amylase activity released into the medium expressed per culture. B: amylase release expressed as percentage of total amylase activity initially present in the cultures.

From Logsdon 48
Figure 5. Figure 5.

Electron micrographs of AR42J cells. A: electron micrograph of a typical AR42J cell cultured in the absence of dexamethasone. Cell displays an undifferentiated appearance. Structural specializations for secretion are not present. Bar, 10 μm. X 10,400. B: electron micrograph of a typical AR42J cell cultured for 48 h in the presence of 10 nM dexamethasone. Cell has secretory granules and abundant rough endoplasmic reticulum. Bar, 10 μm. X 11,600.

From Logsdon et al. 50, by copyright permission of the Rockefeller University Press
Figure 6. Figure 6.

Time course of dexamethasone‐induced increase in 125I‐labeled CCK binding to AR42J cells. AR42J cells were cultured in the presence of dexamethasone 10 nM for up to 72 h) and were then incubated with 30 pM 125I‐labeled CCK for 1 h at 22°C. Values are percent total 125I‐labeled CCK bound per milligram of protein and are means +L SE.

From Logsdon 48
Figure 7. Figure 7.

Effect of increasing insulin concentrations on amylase levels in AR42J cells. Cells were plated onto 35‐mm dishes at a density of 4–6 X 105 cells/dish. After 24 h, media were removed and fresh medium either without or with increasing concentrations of insulin was added. After a further 24 h, medium and hormone concentrations were replaced, and amylase levels were determined the following day.

From Mössner et al. 69
Figure 8. Figure 8.

Dose response for effects of caerulein on incorporation of [3H]thymidine into pancreatic monolayer cultures. Cells were plated for 24 h in basal medium, then changed to basal medium plus indicated concentrations of caerulein for 3 days, the last 24 h of which included 0.1 SmUCi of [3H]thymidine. Values are percent incorporation measured in cultures maintained in basal media and are means +L SE of means for 3–6 experiments.

From Logsdon and Williams 55
Figure 9. Figure 9.

Time dependence of decrease in specific [N‐methyl‐3H]‐scopolamine ([3H]NMS) binding to guinea pig pancreatic acini cultured with 0.1 mM carbachol (CCh) (open circles). Acini cultured in hormonally defined medium without CCh (filled circles). At each time indicated, acini were rinsed with N‐2‐hydroxyethylpiperazine‐N'‐2‐ethanesulfonic acid (HEPES)‐buffered Ringer's solution and incubated for 60 min at 37°C in HEPES‐buffered Ringer's solution containing 0.5 nM [3H]NMS. Results represent means +L SE of 3 experiments.

From Hootman et al. 32


Figure 1.

Light micrograph of cross section of 14‐day‐old pancreatic cultures on collagen gels. Cells form a monolayer of cuboidal cells with obvious polarity. Bar, 20 μm. X 1,100.

From Logsdon and Williams 55


Figure 2.

Electron micrograph of a typical cell from a 14‐day‐old pancreatic culture in cross section. Bar, 5 μm. X 3,600.

From Logsdon and Williams 55


Figure 3.

Time course of the dexamethasone‐induced increase in amylase concentration in AR42J cells. Dexamethasone 10 nM) was incubated with AR42J cells for up to 72 h. Cells were then analyzed for contents of amylase and protein. Values are units of amylase per milligrams of total protein and are means +L SD.

From Logsdon et al. 50, by copyright permission of the Rockefeller University Press


Figure 4.

Concentration dependence of amylase release stimulated by cholecystokinin octapeptide (CCK‐8) for AR42J cells raised for 48 h in the absence (open circles) or presence (filled circles) of 10 nM dexamethasone. Amylase release of 40 min is plotted as a function of concentration of CCK‐8 in the medium. A: amylase activity released into the medium expressed per culture. B: amylase release expressed as percentage of total amylase activity initially present in the cultures.

From Logsdon 48


Figure 5.

Electron micrographs of AR42J cells. A: electron micrograph of a typical AR42J cell cultured in the absence of dexamethasone. Cell displays an undifferentiated appearance. Structural specializations for secretion are not present. Bar, 10 μm. X 10,400. B: electron micrograph of a typical AR42J cell cultured for 48 h in the presence of 10 nM dexamethasone. Cell has secretory granules and abundant rough endoplasmic reticulum. Bar, 10 μm. X 11,600.

From Logsdon et al. 50, by copyright permission of the Rockefeller University Press


Figure 6.

Time course of dexamethasone‐induced increase in 125I‐labeled CCK binding to AR42J cells. AR42J cells were cultured in the presence of dexamethasone 10 nM for up to 72 h) and were then incubated with 30 pM 125I‐labeled CCK for 1 h at 22°C. Values are percent total 125I‐labeled CCK bound per milligram of protein and are means +L SE.

From Logsdon 48


Figure 7.

Effect of increasing insulin concentrations on amylase levels in AR42J cells. Cells were plated onto 35‐mm dishes at a density of 4–6 X 105 cells/dish. After 24 h, media were removed and fresh medium either without or with increasing concentrations of insulin was added. After a further 24 h, medium and hormone concentrations were replaced, and amylase levels were determined the following day.

From Mössner et al. 69


Figure 8.

Dose response for effects of caerulein on incorporation of [3H]thymidine into pancreatic monolayer cultures. Cells were plated for 24 h in basal medium, then changed to basal medium plus indicated concentrations of caerulein for 3 days, the last 24 h of which included 0.1 SmUCi of [3H]thymidine. Values are percent incorporation measured in cultures maintained in basal media and are means +L SE of means for 3–6 experiments.

From Logsdon and Williams 55


Figure 9.

Time dependence of decrease in specific [N‐methyl‐3H]‐scopolamine ([3H]NMS) binding to guinea pig pancreatic acini cultured with 0.1 mM carbachol (CCh) (open circles). Acini cultured in hormonally defined medium without CCh (filled circles). At each time indicated, acini were rinsed with N‐2‐hydroxyethylpiperazine‐N'‐2‐ethanesulfonic acid (HEPES)‐buffered Ringer's solution and incubated for 60 min at 37°C in HEPES‐buffered Ringer's solution containing 0.5 nM [3H]NMS. Results represent means +L SE of 3 experiments.

From Hootman et al. 32
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Article Title: Long‐Term Regulation of Pancreatic Function Studied in Vitro
 

How to Cite

Craig D. Logsdon. Long‐Term Regulation of Pancreatic Function Studied in Vitro. Compr Physiol 2011, Supplement 18: Handbook of Physiology, The Gastrointestinal System, Salivary, Gastric, Pancreatic, and Hepatobiliary Secretion: 515-530. First published in print 1989. doi: 10.1002/cphy.cp060326